# Cis-regulation of EGFR in glioblastoma

> **NIH NIH F30** · CASE WESTERN RESERVE UNIVERSITY · 2020 · $50,520

## Abstract

PROJECT SUMMARY/ABSTRACT
Glioblastoma is the most common primary intrinsic brain tumor and universally lethal. Like many other
cancers, glioblastomas have recurrent focal amplifications of oncogenes that promote cancer growth. It is
not known to what extent other constituents of these amplicons support tumor formation. Cis-regulatory
elements, including enhancers, are vital to the transcriptional regulation of many genes and may be an
underappreciated part of these amplicons. I now propose to study the role of cis-regulatory elements in the
regulation of EGFR, a very commonly amplified oncogene in glioblastoma. To accomplish this goal, I will
use a CRISPR interference (CRISPRi) strategy to selectively repress putative enhancers at the EGFR
locus and assess their role in EGFR regulation and overall cancer cell growth phenotype. Secondly, I will
use chromosome conformation capture strategies to uncover how the looping of cis-regulatory elements in
focal amplifications contributes to EGFR expression. Collectively, these proposed studies will provide a
more general basis for how the cis-regulatory landscape is utilized by oncogenes in focal amplifications.

## Key facts

- **NIH application ID:** 9971338
- **Project number:** 5F30CA236313-02
- **Recipient organization:** CASE WESTERN RESERVE UNIVERSITY
- **Principal Investigator:** Andrew Robert Morton
- **Activity code:** F30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $50,520
- **Award type:** 5
- **Project period:** 2019-07-01 → 2021-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9971338

## Citation

> US National Institutes of Health, RePORTER application 9971338, Cis-regulation of EGFR in glioblastoma (5F30CA236313-02). Retrieved via AI Analytics 2026-05-28 from https://api.ai-analytics.org/grant/nih/9971338. Licensed CC0.

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