# UMass Mouse Metabolic Phenotyping Center - Metabolism Core

> **NIH NIH U2C** · UNIV OF MASSACHUSETTS MED SCH WORCESTER · 2020 · $335,000

## Abstract

Project Summary - Abstract
The Metabolism Core is designed to conduct in vivo and physiological experiments in conscious mice for the
purpose of detailed metabolic phenotyping of transgenic mouse models useful in understanding obesity, diabetes
and its complications. The Core operates under the supervision of Dr. Jason Kim (Director) and Drs. Roger Davis
and Hye-Lim Noh as co-Directors who oversee day-to-day operation of the Core and interact with the users of
the UMass MMPC. The principal functions of the Core are to perform the following experiments: 1)
hyperinsulinemic-euglycemic clamp to assess organ-specific insulin action and glucose metabolism in conscious
mice, 2) hyperglycemic clamp to assess in vivo pancreatic β-cell function in conscious mice, 3) glucose/insulin
tolerance tests, 4) survival surgery for in vivo experiments, 5) implementation of osmotic pumps for chronic
delivery of drugs or other agents, 6) application of special diet (e.g., high-fat diet) to alter energy balance and
induce obesity in mice, 7) acute lipid infusion, acute/chronic delivery of cytokines, and STZ injection to alter
glucose homeostasis, 8) non-invasive measurement of energy expenditure, respiratory exchange ratio, physical
activity, and food/water intake in conscious mice using metabolic cages, 9) non-invasive assessment of whole
body, organ, and tissue biopsy composition of fat/lean/water mass in conscious mice using 1H-MRS, 10)
implementation of treadmill and cage wheels for exercise study, 12) biochemical analysis for intracellular
metabolic flux measurement (glycolysis, glycogen synthesis), and 13) blinded drug trial studies. The Metabolism
Core regularly interacts with the Analytical and Functional Core for complementary, high-throughput analytical
assays to measure hormones, cytokines and metabolites using serum samples obtained at the end of metabolic
experiments. The goal of the Metabolism Core is to use an array of elegant, non-invasive and in vivo experiments
to obtain a robust set of metabolic data from individual mouse in order to provide mechanistic insights into the
role of selected gene on obesity, insulin resistance, and diabetes.

## Key facts

- **NIH application ID:** 9972927
- **Project number:** 5U2CDK093000-10
- **Recipient organization:** UNIV OF MASSACHUSETTS MED SCH WORCESTER
- **Principal Investigator:** JASON K KIM
- **Activity code:** U2C (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $335,000
- **Award type:** 5
- **Project period:** — → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9972927

## Citation

> US National Institutes of Health, RePORTER application 9972927, UMass Mouse Metabolic Phenotyping Center - Metabolism Core (5U2CDK093000-10). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9972927. Licensed CC0.

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