# UMass Mouse Metabolic Phenotyping Center - Analytical and Functional Core

> **NIH NIH U2C** · UNIV OF MASSACHUSETTS MED SCH WORCESTER · 2020 · $251,250

## Abstract

Project Summary - Abstract
The primary objective of the Analytical & Functional Core is to conduct standardized and specialized experiments
in conscious mice and derived tissues for the comprehensive metabolic characterization of mouse models of
obesity, diabetes and its complications. The Core consists of 3 sub-Cores that are complimentary in nature. The
Analytical sub-Core is directed by Dr. Randall Friedline with assistance from Ms. Hu and provides the following
services: 1) High-throughput and multiplexed measurement of serum/tissue/cell culture levels of hormones,
cytokines, and chemokines using Luminex multiplexing technology, 2) High-throughput measurement of
blood/tissue/urine levels of metabolites and electrolytes using a Cobas Clinical Chemistry Analyzer, 3) Molecular
analysis of intracellular signaling pathways associated with insulin action, glucose metabolism, and inflammation,
and 4) Ultra-sensitive single-plex ELISA measurements of difficult to detect analytes or those not available in
multi-plex format. The Islet sub-Core is co-directed by Drs. Laura Alonso and Dale Greiner and provides the
following services: 1) islet architecture using H&E images, 2) β-cell mass, proliferation, death, and size, and 3)
islet function measuring static, perifusion, and in vivo glucose stimulated insulin secretion. The Cardiovascular
sub-Core is co-directed by Drs. John Keaney and Timothy Fitzgibbons and provides the following services: 1)
High-resolution two-dimensional and M-mode echocardiography using VisualSonics Vevo2100 In Vivo Imaging
System to assess left ventricular systolic dimensions and function, left ventricular diastolic dimensions and
function including tissue Doppler imaging, and cardiac valve function, 2) Ultrasound imaging of thoracic aorta
vascular morphology, 3) Non-invasive assessment of blood pressure and heart rate using CODA blood pressure
monitoring system, and 4) Assessment of vascular and endothelial function.

## Key facts

- **NIH application ID:** 9972928
- **Project number:** 5U2CDK093000-10
- **Recipient organization:** UNIV OF MASSACHUSETTS MED SCH WORCESTER
- **Principal Investigator:** Laura C Alonso
- **Activity code:** U2C (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $251,250
- **Award type:** 5
- **Project period:** — → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9972928

## Citation

> US National Institutes of Health, RePORTER application 9972928, UMass Mouse Metabolic Phenotyping Center - Analytical and Functional Core (5U2CDK093000-10). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9972928. Licensed CC0.

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