# Antibody and Phage Display Shared Resource

> **NIH NIH P30** · COLD SPRING HARBOR LABORATORY · 2020 · $238,166

## Abstract

Antibody and Phage Display Shared Resource - Project Summary/Abstract
The Antibody Shared Resource provides a central site for the production of monoclonal and polyclonal
antibodies. The purpose of this Shared Resource is to provide the resources and expertise to generate these
valuable reagents in a highly efficient and cost-effective manner. In the case of monoclonal antibodies, CSHL
Cancer Center scientists provide specific antigens and the Shared Resource personnel handle all stages of
immunization, fusion, primary screening of hybridomas, cell culture, single-cell cloning, freezing and storage of
hybridoma lines, and large-scale production of monoclonal antibodies in cell culture or in vivo. Immunization,
test bleeds, and production of ascites fluids are coordinated with the personnel of the Animal Shared
Resource. Specialized monoclonal antibody screens are closely coordinated with individual scientists.
Polyclonal antibody production is outsourced, but the Shared Resource personnel are in charge of processing
and distributing of samples, thus reducing the costs and maximizing efficiency. The Shared Resource also
tests and implements new techniques, reagents, and assay improvements related to the different stages of
antibody production. After extensive discussions among the Leadership of the Cancer Center, followed by
consultation with the Cancer Center External Advisory Board, it was decided that the inclusion of phage-
display technology would ensure that this important Shared Resource remains at the cutting edge. This
technology provides the capability for rapid production and optimization of high-affinity synthetic antibodies in a
manner to complement existing expertise and services of this Shared Resource. It is anticipated that this step
forward will not only furnish Cancer Center members with essential reagents, but also may open up new
therapeutic opportunities.

## Key facts

- **NIH application ID:** 9975713
- **Project number:** 5P30CA045508-33
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** Adrian R Krainer
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $238,166
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9975713

## Citation

> US National Institutes of Health, RePORTER application 9975713, Antibody and Phage Display Shared Resource (5P30CA045508-33). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9975713. Licensed CC0.

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