# DNA Sequencing Shared Resource

> **NIH NIH P30** · COLD SPRING HARBOR LABORATORY · 2020 · $147,310

## Abstract

DNA Sequencing Shared Resource - Project Summary/Abstract
The DNA Sequencing Shared Resource provides cost effective rapid DNA sequencing to members of the
CSHL Cancer Center and provides access to state-of-the-art Next-generation DNA sequencers (NGS). Over
the last five years, 21 Cancer Center members (57% of members) used NGS services and 34 (92%) used
Sanger sequencing services. Overall, 61% of total use of this Shared Resource was by Cancer Center
members over the last five years, resulting in 116 publications.
The Resource has allowed Cancer Center members who have smaller scale research projects to gain access
to Next-generation sequencing technology, while enabling other members with large exome sequencing
projects to complete their research. The DNA Sequencing Shared Resource supports cancer genome and
exome re-sequencing, de novo cancer genome sequencing, RNA-Seq, Chip-Seq, RIP-Seq, sequencing of
custom gene panels and re-sequencing of single-cell genomes and transcriptomes. The Cancer Center
research supported by the DNA Sequencing Shared Resource during the last five years resulted in 118
publications.
The Shared Resource also provides guidance in the area of experimental design and data analysis, and
training in preparing samples for DNA sequencing. Importantly, it also provides when needed, advanced
technical capabilities developed for specific projects to Cancer Center members. Finally, the Shared
Ressource provides educational opportunities to Cancer Center members to learn about new sequencing
protocols and technologies that can impact their research.
Since 2010, the Resource has acquired two Pacific Biosciences sequencers one Illumina NextSeq, two
Illumina Miseqs, one Illumina HiSeq2500 and four Illumina HiSeq2000s. The DNA Shared Resource has also
developed and deployed a custom made laboratory information management system and upgraded its
computer storage capacity to better serve the Cancer Center members.
In summary, the DNA Sequencing Shared Resource provides access to state-of-the-art DNA sequencing
services at a competitve cost and with a fast turn around time, thereby enabling and enhancing cancer
research at CSHL.

## Key facts

- **NIH application ID:** 9975716
- **Project number:** 5P30CA045508-33
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** William Richard McCombie
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $147,310
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9975716

## Citation

> US National Institutes of Health, RePORTER application 9975716, DNA Sequencing Shared Resource (5P30CA045508-33). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/9975716. Licensed CC0.

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