# Functional Genomics and Genetics Shared Resource

> **NIH NIH P30** · COLD SPRING HARBOR LABORATORY · 2020 · $424,079

## Abstract

Functional Genomics and Genetics Shared Resource - Project Summary/Abstract 
The Functional Genomics and Genetics Shared Resource provides members of the CSHL Cancer Center with 
access to advanced RNAi and CRISPR screening technologies and services deriving genetically engineered 
cells and animals. This Shared Resource was recently established through reorganization and integration of 
services from the Microarray and RNA Quantitation Shared Resource, the Gene Targeting Shared Resource, 
and new technologies for shRNA and CRISPR screening. The recent revolution in sequencing technology has 
facilitated the collection of massive amounts of human genomic and expression data. To understand the 
human cancer genome and reveal therapeutic opportunities, a complementary set of functional genomics tools 
is required to systematically discover pathway relationships among genes. It is the goal of the Functional 
Genomics and Genetics Shared Resource to leverage its innovative approaches to RNAi and genome editing 
to promote discovery of new therapeutic targets and to create disease relevant models for improving human 
health. A major focus of this Shared Resource is in assisting investigators with the design, implementation, and 
data analysis of multi-plexed RNAi/CRISPR screens in mammalian cells. Functional genomic screening is 
primarily provided as a Shared Resource-assisted service with some full-service screening options. In addition, 
the Shared Resource also offers full-service construction of custom RNAi/CRISPR plasmid libraries. In order to 
extend functional assessment of genetic contributions in the context of genetically engineered cells and mice, 
this Shared Resource provides production of genetically modified mice using Cas9 and single guide RNA 
(sgRNA) microinjection into 1-cell mouse zygotes. A range of other gene targeting services, including gene 
targeting in mouse embryonic stem (ES) cells, derivation of ES cells from mouse models, production of 
chimeric mice by blastocyst injection, production of transgenic mice by pronuclear injection, 
cryopreservation/recovery of embryo and sperm, and in vitro fertilization are also available. Re-derivation of 
animals through this Shared Resource is also a key service for maintaining a pathogen-free animal facility. 
This Shared Resource will be vital to the Cancer Center because it will enable scientists to use genetic 
screening tools to discover novel cancer genes, therapeutic targets, and mechanisms of drug resistance and 
for developing new animal models of cancer for pre-clinical studies. Over the last grant period, 18 Cancer 
Center members (49% of the membership) used the combined services of the new Shared Resource.

## Key facts

- **NIH application ID:** 9975719
- **Project number:** 5P30CA045508-33
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** CHRISTOPHER VAKOC
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $424,079
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9975719

## Citation

> US National Institutes of Health, RePORTER application 9975719, Functional Genomics and Genetics Shared Resource (5P30CA045508-33). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9975719. Licensed CC0.

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