# A High-Throughput Molecular Profiling Core for the CaST Center

> **NIH NIH U54** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2020 · $242,280

## Abstract

PROJECT SUMMARY
The Molecular Profiling Core will provide integrated drug screening and high-throughput expression profiling,
single cell RNA-Seq, and basic RNA-Seq and exome sequencing services to every project in the center. Using
our recently developed PLATE-Seq technology, we can produce high-dimensional expression profiles across
every well in a high-throughput drug screen. We will also provide a microfluidic implementation of PLATE-Seq
for large-scale single cell expression profiling which will be used by center investigators to identify phenotypic
subpopulations of tumor cells that give rise to drug resistance. Center investigators also require basic
sequencing services including deep exome and RNA-Seq to identify mutations for targeted profiling at the
single cell level and gene fusions. The Core will provide tailored and cost effective library preparation and
sequencing services to facilitate these efforts.

## Key facts

- **NIH application ID:** 9976482
- **Project number:** 5U54CA209997-05
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Peter Alan Sims
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $242,280
- **Award type:** 5
- **Project period:** — → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9976482

## Citation

> US National Institutes of Health, RePORTER application 9976482, A High-Throughput Molecular Profiling Core for the CaST Center (5U54CA209997-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9976482. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*