# Cell Biology and Bioimaging Core

> **NIH NIH P30** · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · 2020 · $155,695

## Abstract

Cutting edge research in the field of metabolic disease requires access to modern imaging, flow
cytometry, and specimen preparation methodology. The COBRE Cell Biology and Bioimaging
Core (CBB Core) is an integrative component within the proposed Phase III cores –
Transgenics, Genomics, and Cell Biology and Bioimaging. The CBB Core provides investigators
access to the instrumentation, training, and expertise needed to conduct cellular and tissue level
phenotyping of nutritionally-modulated or genetically-manipulated models of obesity, diabetes,
or other pathologies of metabolic syndrome. The CBB Core enables both junior and senior level
faculty to explore novel approaches that include quantitative histology and tissue specific
transcript profiling via laser microdissection to increase their competitiveness in an era of
shrinking federal funding. The Core's current capabilities include: widefield and confocal
imaging of fixed and live specimens, total internal reflection fluorescence (TIRF), fluorescent
resonant energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), whole
slide scanning, analytical and preparative flow cytometry, laser microdissection, and histology
services that encompass tissue processing, embedding, sectioning, and staining – routine,
special, and immunohistochemical. The Core continually adapts to the changing needs of the
COBRE investigators through frequent self-assessments and oversight by the External Advisory
Committee and the proposed Internal Steering Committee headed by the Program Coordinator,
Dr. Claude Bouchard. The sustainability of the Core is likely due to the high level of commitment
by the PBRC Administration as well as increases in the number of COBRE investigators that will
receive extramural funding. The CBB Core aims are: 1) To provide cost effective specimen
preparation and produce high quality bioimaging and flow cytometry data; 2) To provide access,
training, and assistance with CBB Core platforms, in concert with expertise in application of the
requisite software algorithms needed to produce state-of-the art data analysis, interpretation,
and presentation; 3) To promote development new techniques, technologies, and experimental
approaches needed to enhance COBRE faculty competitiveness for extramural funding.

## Key facts

- **NIH application ID:** 9978086
- **Project number:** 5P30GM118430-05
- **Recipient organization:** LSU PENNINGTON BIOMEDICAL RESEARCH CTR
- **Principal Investigator:** David H Burk
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $155,695
- **Award type:** 5
- **Project period:** 2016-08-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9978086

## Citation

> US National Institutes of Health, RePORTER application 9978086, Cell Biology and Bioimaging Core (5P30GM118430-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9978086. Licensed CC0.

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