# National Gnotobiotic Rodent Resource Center

> **NIH NIH P40** · UNIV OF NORTH CAROLINA CHAPEL HILL · 2020 · $539,352

## Abstract

Project Summary:
Environmental factors, especially microbiota, modify genetic susceptibility to many chronic diseases. The
NGRRC provides an essential resource for local, regional and national multidisciplinary investigators to explore
the hypothesis that residental bacteria strongly influence physiologic processes in normal hosts and
pathogenic inflammatory, metabolic and neoplastic responses in genetically susceptible hosts. This unit
provides a resource for broadly based NIH-funded investigators to examine physiologic and pathophysiologic
differences in germ-free (GF, sterile) vs. gnotobiotic (selectively colonized) vs. specific pathogen free mice of
different genetic backgrounds, to explore the functional alterations of normal vs. dysbiotic microbiota in murine
models and human diseases, and to define the functional relevance of bacterial genes. The microbiota can be
precisely manipulated by colonizing GF mice with single or multiple resident or pathogenic bacterial, viral or
fungal strains using isogenic wild type or mutated strains. In addition, fecal transplants can be performed from
murine models or human donors. Aims: 1. Provide GF and gnotobiotic WT and mutant mice, their tissues and
cells to NIH-funded investigators. 2. Derive additional GF mouse strains for NIH -funded investigators. 3.
Support pilot studies for new investigators to generate preliminary data for NIH grant applications. 4. Train
personnel to develop murine and zebrafish gnotobiotic facilities in other institutions. We provide a unique and
essential resource for a multidisciplinary group of NIH-funded investigators to study physiologic and patho-
physiologic functions of normal and dysbiotic resident bacteria, with particular emphasis on gene/environ-
mental interactions in genetically altered gnotobiotic mice (transgenic, knockout or spontaneously mutated)
with altered physiology and disease phenotypes. In the past 5 years of funding, the NGRRC provided 8279
gnotobiotic mice to 101 investigators in 49 institutions. 68 funded NIH grants (in 12 Institutes) by 51
investigators as well as 63 grants by other funding agencies depend on gnotobiotic mice from the NGRRC.
New directions include providing consultation and training for use of gnotobiotic zebrafish and technical
support for a new gnotobiotic pig facility at NC State Univ. Vet School.

## Key facts

- **NIH application ID:** 9978149
- **Project number:** 5P40OD010995-17
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **Principal Investigator:** Ryan B Sartor
- **Activity code:** P40 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $539,352
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9978149

## Citation

> US National Institutes of Health, RePORTER application 9978149, National Gnotobiotic Rodent Resource Center (5P40OD010995-17). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9978149. Licensed CC0.

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