# REACTIVE OXYGEN SPECIES CORE

> **NIH NIH P01** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2020 · $290,489

## Abstract

PROJECT SUMMARY
The purpose of REACTIVE OXYGEN SPECIES CORE A is to provide state-of-the-art measurements of
reactive oxygen species (ROS), identify the sources of ROS and assist with ROS studies in intact cells and
cellular fractions, ex vivo and in vivo for participants of the PPG. Core A will assist with preparation and
processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy,
High Performance Liquid Chromatography (HPLC) and fluorescent spectroscopy. This Core facility has
 
supported numerous studies of superoxide (O2 ), hydrogen peroxide (H2O2) and NO in vascular cells and
tissue. This facility will significantly enhance the work of the individual laboratories and will enhance
collaborations between participants of the PPG. Specifically, the Core A will:
1. Offer expertise, facilities, and training for individuals in performing measurements of ROS, NO and
 peroxynitrite using ESR spectroscopy, HPLC and fluorescent spectroscopy.
2. Provide assistance with state-of-the-art in vitro, ex vivo and in vivo measurements of ROS, NO and
 peroxynitrite in cells and tissues for participants of the PPG.
3. Develop techniques and refine existing methods for measurement of ROS, NO and peroxynitrite in cells
 and tissues.
The core leader, Dr. Dikalov, is an expert in in vitro and ex vivo measurements of ROS and NO in vascular
cells and tissues and has been at the forefront in developing new methodologies for ROS detection. Much of
his work has been focused on development of new ESR and HPLC based techniques for in vitro and in vivo
measurements of ROS. REACTIVE OXYGEN SPECIES CORE A will develop new methods and refine existing
techniques for measurements of ROS in cells and relevant tissues using ESR probes, boronate-based
peroxynitrite fluorescent probes and redox sensitive GPF fluorescent protein targeted to specific intracellular
organelles. The application of these methods will enhance the ability of the individual project leaders to test
their hypotheses and design new studies as necessary to advance their work.

## Key facts

- **NIH application ID:** 9978624
- **Project number:** 5P01HL129941-05
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** Sergey Dikalov
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $290,489
- **Award type:** 5
- **Project period:** 2016-08-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9978624

## Citation

> US National Institutes of Health, RePORTER application 9978624, REACTIVE OXYGEN SPECIES CORE (5P01HL129941-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9978624. Licensed CC0.

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