# Mechanisms of T cell Activation in Hypertension

> **NIH NIH P01** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2020 · $571,206

## Abstract

ABSTRACT
In recent years, research from our laboratory and others has shown that innate and adaptive immunity play
critical roles in the genesis of hypertension. The major hypothesis of this project is that the central nervous
system coordinates actions of dendritic cells (DCs) and T cells, which in turn affect the vasculature and kidney
to raise blood pressure. We have also discovered a novel role of isoketal-protein adducts in hypertension.
These oxidatively modified proteins accumulate in DCs, lead to DC activation and seem to act as neoantigens.
In a recent study, we have also shown that a major site of DC and T cell activation in hypertension is the
kidney. These findings are extremely novel, because they show a new role of DCs in hypertension, and we
plan to gain further insight into activation of these cells by hypertensive stimuli in vivo. We will interrupt
sympathetic outflow by deleting the NADPH oxidase subunit p22phox in the rostroventral lateral medulla using
cre-lox technology and will prevent the central actions of angiotensin II by deleting the AT1 receptor (AT1R) in
circumventricular organs. We will also examine the direct effect of angiotensin II on DCs by deleting the AT1R
in DCs, again using Cre-Lox approaches. The impact of these interventions will be monitored by examining DC
isoketal-adduct formation and superoxide production in conjunction with Core A. In aim 2, we will employ novel
mice we have made that have soluble forms of class 1 major histocompatibility complexes (MHC1). DCs from
these mice shed large quantities of MHC1 and we have adopted methods to harvest isoketal-adducted
peptides from these for proteomic analysis. In collaboration with project 3, we will examine the ability of specific
isoketal-adducted peptides identified in aim 2 to drive proliferation of hypertension-specific T cell hybridomas.
In aim 3, we will examine the role of memory T cells in responses to repeated hypertensive challenges. Our
preliminary data show that these have a critical role in modulating salt-sensitive hypertension. A model
developed in conjunction with project 3 will be employed. Preliminary data indicate that these repeated
challenges promote renal accumulation of effector memory T cells (TEM cells) that produce large amounts of IL-
17A and IFN-. We also find that TEM cells accumulate in the bone marrow of hypertensive mice, and will
determine the role of these in responding to repeated hypertensive stimuli, and how the sympathetic nervous
system modulates their trafficking and activation. An important aspect of this aim will be to determine if
memory T cells of humans with either hypertension or pre-hypertension have epigenetic alterations that
predispose to inflammatory cytokine production. These experiments will employ a novel ATAC-seq technology,
performed in collaboration with Drs. Weyand and Goronzy in project 2, to map the epigenetic landscape of T
cell subsets. Overall, these studies promise to further our understanding...

## Key facts

- **NIH application ID:** 9978625
- **Project number:** 5P01HL129941-05
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** David G Harrison
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $571,206
- **Award type:** 5
- **Project period:** 2016-08-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9978625

## Citation

> US National Institutes of Health, RePORTER application 9978625, Mechanisms of T cell Activation in Hypertension (5P01HL129941-05). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/9978625. Licensed CC0.

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