# TRD1

> **NIH NIH P41** · UNIVERSITY OF CALIFORNIA-IRVINE · 2020 · $333,824

## Abstract

Continuing support is proposed for the Laboratory for Fluorescence Dynamics to expand
technological biomedical research to new fields of discovery and applications and to strengthen
collaborative, service, training and dissemination activities for the benefit of the biomedical
research community. Fluorescence imaging and fluorescence methodologies have reached a
prominent role in Biology, Biophysics and in Clinical Research. The LFD is a biotechnological
resource center with a history of successes in developing new fluorescence technologies for
biology and medicine and with a strong commitment to service, training, and dissemination
components aimed at bringing fluorescence methods in a user facility available to the
biomedical research community.
For this proposal we have conceived a strong technical development program to expand current
limits of imaging fluorescence instrumentation, particularly for the study of dynamical cellular
processes. Projects include: developments of new microscope platforms for imaging in deep
tissues, still maintaining diffraction limited resolution; exploiting the light sheet microscope for
fluctuation analysis with the potential for developing radical new approaches to measure
molecular flow in cells and tissue at the nanoscale; using new electronics and optics for 3D
imaging of cellular dynamics; expanding the phasor approach to fluorescence lifetime imaging
analysis to spectral and diffusion analysis for fast, fit-free visualization of dynamical processes
and expanding our software to allow new algorithms for data acquisition and analysis in our new
microscopes.
The organization structure of the LFD allows users and collaborators to access innovative
technologies only available in this BTRR resource, which affords them a unique opportunity to
rapidly advance their own research programs. A rich program of driving biological problems
exploit and push technological advances and research in biological processes, macromolecular
assembly, tissue organization and dynamics, membrane morphology/function relationships, and
innovative biomedical instrumentation for detection of bacteria in blood which could have an
impact in the clinical area. Educational programs proposed for this application include hands-on
training of students, postdoctoral fellows and visiting scientists, workshops and specialized
schools in innovative fluorescence methodologies. Our improved web site will continue to be a
reference source for information about fluorescence dynamic techniques, and a large repository
of publications, lectures and tutorials.

## Key facts

- **NIH application ID:** 9978897
- **Project number:** 5P41GM103540-35
- **Recipient organization:** UNIVERSITY OF CALIFORNIA-IRVINE
- **Principal Investigator:** ENRICO GRATTON
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $333,824
- **Award type:** 5
- **Project period:** 1997-08-01 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9978897

## Citation

> US National Institutes of Health, RePORTER application 9978897, TRD1 (5P41GM103540-35). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9978897. Licensed CC0.

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