# Research Project 3

> **NIH NIH P01** · FLORIDA GULF COAST UNIVERSITY · 2020 · $132,064

## Abstract

Project Summary
P01 Grant Title: Greater Caribbean Center for Ciguatera Research
Project 3 title: Translation: Human mechanisms of genotoxicity and cellular metabolism
Project 3 inter-relates with Projects 1 and 2 by providing details of human cellular response following exposure to
the isolated Ciguatoxins. Discoveries herein will be shared with the Project leads and Administrative core to
provide a comprehensive information flow to the Community Engagement Core for regular content distribution
and awareness to the effected stakeholders. Guided by the modeling of Project 1 to understand
Gambierdiscus population dynamics in the region, Project 2 will yield the isolated Gambierdiscus-derived toxins,
including details on bioaccumulation throughout the foodweb. The Project 3 team will work closely with the Project
2 lead (Alison Robertson) to clarify and discover genotoxicity mechanisms of CTX exposure, how exposure varies
(acute vs chronic) and if exposure impacts secondary exposure to other environmental agents. Finally, Project 3
will evaluate detailed mechanisms of subcellular crosstalk that may reveal novel biomarkers associated with
exposure. This will be accomplished, as will be described in detail throughout the Research Plan, using novel,
state-of-the-art technologies developed by and optimized in the Sobol lab, including a new genotoxin screening
platform (CometChip) that provides greater than ~10,000-fold greater capacity and sensitivity than previous
genotoxicity analytical approaches, novel high-resolution cellular imaging tools for Ca2+ signaling, metabolite
(ATP, NAD+) quantification, cell death mechanisms, mitochondrial function and genomic signaling response.

## Key facts

- **NIH application ID:** 9979881
- **Project number:** 5P01ES028949-03
- **Recipient organization:** FLORIDA GULF COAST UNIVERSITY
- **Principal Investigator:** Robert W Sobol
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $132,064
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9979881

## Citation

> US National Institutes of Health, RePORTER application 9979881, Research Project 3 (5P01ES028949-03). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9979881. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*