# Core D - Pathology and Flow Cytometry Core

> **NIH NIH P01** · UNIVERSITY OF LOUISVILLE · 2020 · $307,107

## Abstract

The function of the Flow Cytometry and Pathology (Core D) is to provide each Project with the expertise,
equipment, and facilities required for the flow cytometric analyses, cell sorting, and pathological analyses that
are needed for studies of cardiac mesenchymal cells (CMCs) and extracellular vesicles (EVs). Accurate
identification, characterization, and quantitation of cells in a complex heterogeneous population are technically
demanding and require specialized and expensive equipment and personnel. One of the major advantages of
this Program Project is that it will make available to all four Projects the facilities and techniques of flow
cytometry and cell sorting, which otherwise would not be affordable to any Project in isolation. Flow cytometry-
based analysis for the identification of specific cell populations, changes in cell commitment or phenotype, and
characterization of cell functions such as cell cycle, viability, or signal transduction, will be crucial to all
Projects. A central common facility that performs these technically challenging analyses is therefore
indispensable to achieve the goals of the Program Project. Besides the obvious advantages of efficiency and
cost savings, the Core will also enable four investigators with diverse and complementary backgrounds (Drs.
O’Toole, Moore, Li, and Ratajczak) to combine their technical and biological expertise in order to ensure that
the proposed work is successfully executed. The Core will offer the Program Project investigators the ability to
identify and characterize specific cell types from complex populations. It will also offer the facilities and
expertise necessary to perform the comprehensive histopathologic/immunohistochemical analyses required for
this Program. The uniform histopathologic analyses conducted by the Core in a blinded and rigorously
standardized fashion will make it possible to compare results of different experiments within the same Project
and among different Projects.
Core D will be the continuation of the Flow Cytometry Core in the current funding cycle (led by Dr. O’Toole)
and of the intense and productive ongoing collaboration in flow cytometry and pathology between Dr. O’Toole
and the other PPG investigators. The function of Core D is to provide all four Projects with the expertise,
equipment, facilities, reagents, procedures, and analyses needed for flow cytometry, sorting, and pathology
work. Specifically, the Core will i) perform the flow cytometric analyses and cell sorting procedures required for
studies of CMCs and EVs, ii) perform tissue processing, histopathology, and immunohistochemistry of cardiac
samples, and iii) make available to the four Projects the tremendous experience and expertise of the Core
personnel.

## Key facts

- **NIH application ID:** 9980482
- **Project number:** 5P01HL078825-14
- **Recipient organization:** UNIVERSITY OF LOUISVILLE
- **Principal Investigator:** Timothy E O'Toole
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $307,107
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9980482

## Citation

> US National Institutes of Health, RePORTER application 9980482, Core D - Pathology and Flow Cytometry Core (5P01HL078825-14). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9980482. Licensed CC0.

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