# FLOW CYTOMETRY CORE

> **NIH NIH P30** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2020 · $93,892

## Abstract

PROJECT SUMMARY - VDDRC FLOW CYTOMETRY CORE
The Flow Cytometry and Cell Sorting Core is an established core for the VDDRC. It was developed in direct
response to member requests and approved by the Executive Committee and the Scientific Advisory Board
with substantial institutional support provided by the University. This core provides access to state-of-the-art
equipment for analytical cytometric analysis, cell counts and viability, and cell sorting, and it provides expert
training and consulting on the use of these methodologies. Forty-five (45) VDDRC members used this core
over the last funding period. The core facility maintains analytical flow cytometers, three cell sorters, an
imaging cytometer, a mass cytometer, a mobile cytometer than can be positioned in any lab, and a high-speed
plate sampler. The core provides both Mac and PC workstations, an extensive toolbox of software for flow
cytometry, and digital backup of data on a secure remote server. Sample biosafety is carefully considered and
policies and practices are in place to safely process samples and tissues up to the BSL-3 level. The staff
provides both group and one-on-one instruction to facilitate development of acquisition and analysis skills for
all users. Trained users can use the analytical cytometers directly on a 24 hour/7 days a week/365 days per
year basis; sorting is an assisted-only service during normal operational hours. The core also has worked with
VDDRC investigators to develop novel sorting techniques for elements of subcellular compartments such as
vesicles. This technically forward work requires custom modification of the sorting cytometer in collaboration
with the manufacturer, and involves a large number of technical components from gradient preparations,
staining, lasers and fluidics, and downstream collection and proteomics analysis. The core works with VDDRC
investigators in cutting edge fields such as Mass Cytometry to identify and analyze rare cells with high-content
measurements. The core maintains an active development program to keep the instrumentation and systems
current, functional, accessible, and easy to use. In fact, a number of our machines have been extensively
customized with four or five lasers and special PMTs to accommodate user protocols. Individual researchers
could not support this type of high-quality cytometry in their own labs due to the high cost of the
instrumentation and maintenance.

## Key facts

- **NIH application ID:** 9980893
- **Project number:** 5P30DK058404-19
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** James E Crowe
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $93,892
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9980893

## Citation

> US National Institutes of Health, RePORTER application 9980893, FLOW CYTOMETRY CORE (5P30DK058404-19). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9980893. Licensed CC0.

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