# Quantification of compartmentalized metabolism in eukaryotic systems

> **NIH NIH R01** · TEMPLE UNIV OF THE COMMONWEALTH · 2020 · $340,775

## Abstract

2. PROJECT SUMMARY ABSTRACT
 Acyl-coenzyme A thioesters (acyl-CoAs) are evolutionarily conserved critical metabolic intermediates that
are made and used in distinct parts of the eukaryotic cell. In different sub-cellular compartments, acyl-CoAs can
be used to meet energy needs, act as signaling molecules, and serve as acyl-donors for post-translational
modifications (PTMs) of proteins. A major challenge remaining unaddressed in the field of bioanalytical chemistry
is the quantitation of the subcellular localized pools of these critical metabolites, especially between the
mitochondria, nucleus, and cytosol where the same metabolite can have distinct functions. We will overcome
this challenge in two aims, leveraging stable isotope labeling strategies, quantitative mass spectrometry,
pharmacology, toxicology, and unique cell biology models. First, we will quantify the kinetics and fate of major
carbon substrates for compartmentalized acyl-CoAs in cells and heart tissue. Second, we will quantify the effect
of perturbing compartment specific processes with histone deacetylase inhibitors, electron transport chain
inhibitors, and genetic models on acyl-CoAs, downstream metabolites, and compartment specific PTMs. This
project will allow the rigorous quantification of distinct compartments of metabolism, and thus, their quantitative
study and manipulation in normal and pathological processes where metabolism goes awry.

## Key facts

- **NIH application ID:** 9980952
- **Project number:** 5R01GM132261-03
- **Recipient organization:** TEMPLE UNIV OF THE COMMONWEALTH
- **Principal Investigator:** Nathaniel W. Snyder
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $340,775
- **Award type:** 5
- **Project period:** 2019-08-01 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9980952

## Citation

> US National Institutes of Health, RePORTER application 9980952, Quantification of compartmentalized metabolism in eukaryotic systems (5R01GM132261-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9980952. Licensed CC0.

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