# Flow Cytometry (FC)

> **NIH NIH P30** · UNIVERSITY OF NEBRASKA MEDICAL CENTER · 2020 · $79,654

## Abstract

Project Summary/Abstract: Flow Cytometry Research Facility (FCRF) 
In 2014 the Flow Cytometry Research Facility (FCRF), formerly the Cell Analysis Facility, was re-branded to 
more accurately reflect the services offered. The FCRF is located in the Durham Research Center at the 
University of Nebraska Medical Center (UNMC) campus. The goal of the FCRF is to provide access to the 
latest instrumentation, leading scientific and technical expertise, with comprehensive flow cytometry support to 
all investigators at the University of Nebraska and the surrounding area. The facility houses two multicolor cell 
sorters with 14 simultaneous fluorescent parameter capabilities, one of which is contained within a class 2A 
biological safety cabinet. There are three analog analysis instruments with exceptional low-end resolution, 
ideal for DNA synthesis measurements. There are two high-end, multi-laser and multi-parameter flow 
cytometers with 14 and 18 simultaneous fluorescent parameters respectively. Both of these instruments are 
equipped with a high-throughput system for automation of 96-well plate analysis. Additionally, the facility has a 
pioneering high content, 9-laser, 30-parameter Fortessa X50 flow cytometer; with access to a suite of novel 
reagents and custom fluorochrome conjugations, the FCRF is in a position to offer world-leading, high-content 
flow cytometry capabilities to investigators at University of Nebraska. 
With the University of Nebraska currently investing and actively expanding its research infrastructure, the 
FCRF will become increasingly valuable to a growing research base over the coming five years. In the past 12 
months there has been a strategic shift in operations at the FCRF, initiating a culture change in how 
researchers access flow cytometry services. This has given the FCRF the operational structure in place to 
support an increase in volume of use at the facility in the coming years and an increase in high-content flow 
cytometry applications.

## Key facts

- **NIH application ID:** 9981649
- **Project number:** 5P30CA036727-34
- **Recipient organization:** UNIVERSITY OF NEBRASKA MEDICAL CENTER
- **Principal Investigator:** CRAIG SEMERAD
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $79,654
- **Award type:** 5
- **Project period:** 1997-09-05 → 2021-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9981649

## Citation

> US National Institutes of Health, RePORTER application 9981649, Flow Cytometry (FC) (5P30CA036727-34). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9981649. Licensed CC0.

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