# Molecular Biology (MB)

> **NIH NIH P30** · UNIVERSITY OF NEBRASKA MEDICAL CENTER · 2020 · $162,520

## Abstract

Project Summary/Abstract: Molecular Biology Shared Resource (MBSR) 
The Molecular Biology Shared Resource (MBSR) was created in 2009 to bring together into one consolidated 
operation the Genomics/DNA Microarray Facility and the Molecular Biology/High-Throughput Screening (HTS) 
Facility. The merger enhanced the communication between the cores, maximized the utilization of staff 
expertise, and raised the quality of services used by representatives of all Cancer Center programs. The 
MBSR offers: 1) next-generation sequencing (Illumina), single-cell genomics (Fluidigm), and Sanger 
sequencing; 2) microarray services (Affymetrix); 3) genome-wide siRNA and small-molecule chemical (>100K) 
HTS screening; and 4) high-content imaging and analysis. Additionally, the facility receives, processes, 
annotates, tracks, and banks all patient human biological material (blood and its derivatives, urine, and 
selected tissues) collected by over ten Fred and Pamela Buffett Cancer Center (FPBCC) collaborative 
registries/tissue banks for use in IRB-approved multidisciplinary research projects. The resource provides free 
consultation to every researcher regarding experimental design, budget estimates, results, and data 
assessment, and is a significant resource for molecular biological reagents, information, and training in 
molecular biological techniques and analysis software. The MBSR is under the direction and oversight of Dr. 
M.A. (Tony) Hollingsworth while the Associate Directors manage the day-to-day activities of each Facility. In 
this regard, David Kelly, Ph.D., oversees the Molecular Biology/HTS Facility located in the Eppley Science 
Hall, and Dr. James Eudy oversees the Genomics/DNA Microarray Facility located in the Durham Research 
Center II, both on the UNMC campus. Six technical staff members are employed in the MBSR with personnel 
extensively cross-trained to perform virtually all services and to operate virtually all instrumentation and 
equipment. The MBSR is the most widely used FPBCC Shared Resource, supporting over 220 biomedical 
laboratories in the UNMC research community, of which approximately 40% are laboratories led by FPBCC 
investigators in the FPBCC research programs (Cancer Genes and Molecular Regulation Program, Molecular 
and Biochemical Etiology Program, and Gastrointestinal Cancer Program). To meet the rapidly changing 
needs of and to inform FPBCC investigators, the MBSR personnel conduct workshops, speak at local 
seminars, attend Cancer Center program meetings and multi-investigator project meetings, and present at 
laboratory meetings. Moreover, they attend national and regional technology conferences to learn the latest 
technologies, techniques, and methods.

## Key facts

- **NIH application ID:** 9981651
- **Project number:** 5P30CA036727-34
- **Recipient organization:** UNIVERSITY OF NEBRASKA MEDICAL CENTER
- **Principal Investigator:** Michael A. Hollingsworth
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $162,520
- **Award type:** 5
- **Project period:** 1997-09-05 → 2021-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9981651

## Citation

> US National Institutes of Health, RePORTER application 9981651, Molecular Biology (MB) (5P30CA036727-34). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/9981651. Licensed CC0.

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