# Mimetic Peptides-Mediated Protection Against Coxiella burnetii Infection

> **NIH NIH R01** · UNIVERSITY OF TEXAS SAN ANTONIO · 2020 · $520,516

## Abstract

Abstract
Coxiella burnetii is an obligate intracellular Gram-negative bacterium that causes acute and chronic Q fever in
humans. It is an understudied category B select agent and can be transmitted via aerosol. The existing
formalin-inactivated phase I whole-cell vaccine (PIV) induces serious side effects in previously sensitized
individuals and no vaccine is commercially available for prevention of human Q fever in the US. Therefore,
creation of a safe and effective vaccine to prevent Q fever remains an important public health and national
biosecurity goal. Our long-term goal is to develop novel approaches for safe and effective immunoprophylaxis
of acute and chronic Q fever. Interestingly, our preliminary studies demonstrated that a peptide mimic of a C.
burnetii phase I lipopolysaccharide (PI-LPS) epitope (m1E41920) conjugated to keyhole limpet haemocyanin
(m1E41920-KLH) conferred protection against C. burnetii infection in both mouse and guinea pig models.
These findings support the utility of m1E41920 as a vaccine candidate to prevent human Q fever. Thus, the
objective of this revised R01 application is to define and optimize a PI-LPS-mimetic peptide and recombinant
protein combination vaccine that confers the same level of protection as the PIV. To achieve this objective, we
propose three specific aims to test the central hypothesis that the combination of m1E41920 with additional
protective peptide mimic of PI-LPS and recombinant C. burnetii protein antigens will confer the same level of
protection as the PIV. Aim 1 will use a C. burnetii aerosol infection mouse model to define and optimize a PI-
LPS-mimetic peptide and recombinant protein combination vaccine that will confer the same level of protection
as the PIV. Aim 2 will determine if the protective epitopes of PIV recognized by the mouse model will confer
adequate protection in a guinea pig aerosol infection model. Aim 3 will use a humanized mouse model to
determine if the protective epitopes of PIV recognized by rodents will confer protection in humans. Upon the
completion of the proposed research, we expect to define the first multivalent vaccine for Q fever targeting
novel neutralization-sensitive epitopes of both PI-LPS and PI protein. This will have significant positive effects
on human health, because it will provide the basic information required to ultimately develop a safe, effective
and tractable vaccine against Q fever.

## Key facts

- **NIH application ID:** 9982219
- **Project number:** 5R01AI134681-04
- **Recipient organization:** UNIVERSITY OF TEXAS SAN ANTONIO
- **Principal Investigator:** Guoquan Zhang
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $520,516
- **Award type:** 5
- **Project period:** 2018-08-13 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9982219

## Citation

> US National Institutes of Health, RePORTER application 9982219, Mimetic Peptides-Mediated Protection Against Coxiella burnetii Infection (5R01AI134681-04). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9982219. Licensed CC0.

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