# Understanding Severe Asthma Using an Experimental Model

> **NIH NIH R01** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2020 · $494,870

## Abstract

Severe asthma is a complex heterogeneous disease, which poorly responds to corticosteroids (CS), the
current standard of care for asthma. In a previous study, we reported that bronchoalveolar lavage (BAL) cells
in >50% of subjects with severe asthma (SA) secrete high levels of IFN-γ from CD4+ T cells despite treatment
of the subjects with high doses of inhaled CS, often combined with oral CS. In a recent follow-up study to
understand the molecular mechanisms that promote a persistent Th1high phenotype in SA, we have shown an
essential role for the transcription factor IRF5 in promoting IL-12 production from lung dendritic cells (DCs) and
macrophages (Mφs) to elicit high levels of IFN-γ production from T cells. In another study published recently,
we show that SA subjects with a Th1high profile harbor higher levels of expression of the Th1-recruiting
chemokine CXCL10 in their airways compared to mild asthma subjects, strongly associated with IFN-γ
expression, frequency of oral CS use, and expression of mast cell-specific proteases. Chromatin
immunoprecipitation (ChIP) assay used to determine high CXCL10 levels in SA revealed recruitment of both
STAT1 to and GR to the CXCL10 promoter with no accompanying suppression of CXCL10 mRNA expression.
While CS were unable to suppress IFN-γ-induced CXCL10 mRNA and protein levels in monocytes, IL-10
completely inhibited CXCL10 gene expression in the context of IFN-γ and CS, an effect not realized in many
severe asthmatics. In new data generated using RNA-seq approaches, we show downregulation of the
negative regulator of TGF-β1-mediated signaling, Smad7, and upregulation of c-Myc, in lungs of mice
subjected to the SA model, which may impact mast cells and airway remodeling. Indeed in our recent report
we have shown a strong correlation between CXCL10 mRNA and mast cell signatures in humans and mice.
Mast cells produce cysteinyl leukotrienes (CysLTs), and our RNA-seq data show that IFN-γ selectively
upregulates expression of the CysLT receptor, CySLTR2, in the lung.
Collectively, these studies lead us to hypothesize that the IRF-5-IFN-γ-CXCL10 axis in the context of CS
induces a feed-forward loop promoting a Th1high phenotype that impacts lung function and airway remodeling
involving mast cells. Although refractory to CS, the Th1high state is sensitive to IL-10.
To test this hypothesis we will:
Aim 1. Establish IRF5 in lung APCs as a sustainer of Th1 (IFN-γ) effector function and identify IRF5 SNPs in
humans.
Aim 2. Determine the role of specific IFN-γ-regulated molecules and mast cells in disease pathogenesis in the
SA model.
Aim 3. Investigate defective IL-10 production in T cells in humans with SA and study inhibition by IL-10 of CS-
refractory CXCL10 gene expression induced by IFN-γ.

## Key facts

- **NIH application ID:** 9982408
- **Project number:** 5R01HL113956-07
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** Anuradha Ray
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $494,870
- **Award type:** 5
- **Project period:** 2013-01-07 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9982408

## Citation

> US National Institutes of Health, RePORTER application 9982408, Understanding Severe Asthma Using an Experimental Model (5R01HL113956-07). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9982408. Licensed CC0.

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