# RNA Aptamers as Probes and Regulators of Coagulation

> **NIH NIH P01** · CHILDREN'S HOSP OF PHILADELPHIA · 2020 · $468,145

## Abstract

Project 3 - Abstract
Three interrelated but independent hypotheses are being tested in this project. A.) Aptamers can serve as
molecular probes to identify and study functionally important exosites on coagulation factor cofactors FVa and
FVIIIa; B.) Aptamers targeting specific regions identified within FV and FVIII can serve as procoagulants by
allosterically activating FV or preventing the binding of FVIII to vWF; and C.) Exosite binding Factor V and VIII
aptamers will limit thrombosis in vivo and antidote oligonucleotides can be made to rapidly reverse the
anticoagulant activity of such aptamers to restore normal hemostasis in animals. Our specific aims are:
Aim 1: To generate anticoagulant aptamers against coagulation factors Va and VIIIa and use them as
novel probes for exosite interactions with the cofactors within the respective enzyme complexes.
Aim 2: To generate novel procoagulant aptamers targeting the procofactor FV and FVIII
Aim 3: To evaluate the ability of aptamers targeting exosites on Factor V/Va and VIII/VIIIa to act as
potent yet safe antithrombotic agents.

## Key facts

- **NIH application ID:** 9982420
- **Project number:** 5P01HL139420-03
- **Recipient organization:** CHILDREN'S HOSP OF PHILADELPHIA
- **Principal Investigator:** BRUCE ALAN SULLENGER
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $468,145
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9982420

## Citation

> US National Institutes of Health, RePORTER application 9982420, RNA Aptamers as Probes and Regulators of Coagulation (5P01HL139420-03). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/9982420. Licensed CC0.

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