# Flow Cytometry Core Shared Resource

> **NIH NIH P30** · NORTHWESTERN UNIVERSITY · 2020 · $177,398

## Abstract

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ABSTRACT – FLOW CYTOMETRY CORE FACILITY
The Robert H. Lurie Comprehensive Cancer Center Flow Cytometry Core Facility provides comprehensive flow
cytometry and cell sorting services for investigators of the Lurie Cancer Center and Northwestern University. Its
central mission is to provide guidance, technical assistance, and equipment for investigators to utilize routine and
complex multi-parametric, multi-laser measurements as well as cell sorting, regardless of their level of cytometry
expertise. The Flow Cytometry Core Facility serves individuals interested in cellular-based measurements and
cellular heterogeneity in malignancies, providing critical support for cancer-related research. Services provided
by the facility extend from consultation on experimental design, sample preparation and data analysis to
instrument operation and set-up for cell sorting and multi-laser operation. The Core also has an active program
of flow cytometry protocol and assay development guided by the interests and needs of the investigators. The
Core has extensive experience in developing and implementing complex multi-parametric assays with particular
expertise in intracellular labile antigen detection such as phosphorylated protein epitopes. User training for
operation of analysis and cell sorting instruments gives users around-the-clock access and allows the maximum
use of the Core’s instruments.
The Flow Cytometry Core Facility serves both the Chicago and Evanston campuses. The Chicago campus facility
has 5 cell sorters: Two 5 laser/15-17 parameter BD FACSAria SORP cell sorters, a 6 laser/15-17 parameter BD
FACSAria SORP cell sorter, a 3 laser/10-12 parameter BD FACSAria SORP cell sorter and a 3 laser/10
parameter BD FACSMelody cell sorter. The Chicago campus facility also has three benchtop flow cytometry
analyzers; 6 laser/18 parameter BD LSRFortessa SORP, another 6 laser/18 parameter BD LSRFortessa, which
is being upgraded to 30 parameters and a soon to be purchased Apogee Micro-Plus flow cytometer for small
particle analysis. In addition, the Chicago facility has a Nexcelom cell counter/viability instrument (Nexcelom
Bioscience, LLC). On the Evanston campus, we have one 4 laser, 13 parameter BD FACSAria IIu and a 4
laser/13 parameter BD LSRII. Both facilities have multiple offline analysis workstations providing the components
and all advanced software for post-acquisition data analysis and graphics generation. The Core is heavily utilized
by a wide number of investigators (207) with 63% who are Cancer Center members.

## Key facts

- **NIH application ID:** 9982859
- **Project number:** 5P30CA060553-26
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **Principal Investigator:** Harris R Perlman
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $177,398
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9982859

## Citation

> US National Institutes of Health, RePORTER application 9982859, Flow Cytometry Core Shared Resource (5P30CA060553-26). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9982859. Licensed CC0.

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