# High Throughput Analysis Laboratory Shared Resource

> **NIH NIH P30** · NORTHWESTERN UNIVERSITY · 2020 · $124,646

## Abstract

ABSTRACT – HIGH THROUGHPUT ANALYSIS LABORATORY 
The High Throughput Analysis laboratory (HTA) enables LCC researchers to develop, perform, and analyze 
large scale experiments that elucidate the fundamental biology of cancer and launch the discovery of novel 
therapeutic agents. Such projects can involve parallel manipulation of tens of thousands of samples as well as 
data collection and analysis at a similar scale. To support this work the facility provides advanced technology, 
technical expertise, and resources like compound and gene disruptor libraries for diverse high throughput 
screens (HTS) on biochemical targets and cell processes. HTA staff partner with LCC researchers to adapt 
bench-scale studies into assays that are tractable for such large-scale experiments, and to use these assays to 
run screens. HTA capabilities also support non-HTS projects requiring the use of robotic liquid handling and 
plate readers. 
LCC members use a wide range of experimental systems and technical approaches in their research. HTA is 
therefore configured to develop and run assays ranging from fundamental biochemistry to cell-based studies 
involving high throughput confocal microscopy (known as high content screening or “HCS”). The facility 
maintains a suite of instruments for large-scale experiments that include advanced liquid handling platforms for 
nanoliter to microliter volumes, a diverse set of high-end photometric systems, and two different platforms for 
high-content screening (one of which is a new confocal instrument). The facility’s staff acquires and analyzes 
data from all instruments as a service, assists with experimental design, and trains researchers to use its 
analytical and acquisition software independently. Importantly, HTA is a fully-equipped conventional laboratory 
for cell and molecular biology with significant bench space and a newly renovated in-house tissue culture facility. 
Users can run complex experiments entirely in the facility. The number of LCC research groups supported by 
HTA instruments and services during the most recent one-year reporting period is more than double that reported 
in the previous CCSG renewal application. The increased usage of the facility corresponds to a significant 
expansion of its instrumentation, services and screening resources. This has included significant compound 
library acquisitions and newly developed capabilities for multiplexed and parallelized CRISRP/Cas9 screening 
for large scale functional analysis. HTA actively maintains a productive collaboration with ChemCore, speeding 
the cycle of lead optimization and ensuring that compound library selection maximizes the potential for medicinal 
chemistry with screening hits. Moreover, HTA works with the Developmental Therapeutics Core to develop new 
assays that use advanced tissue culture approaches.

## Key facts

- **NIH application ID:** 9982860
- **Project number:** 5P30CA060553-26
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **Principal Investigator:** CURT M HORVATH
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $124,646
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9982860

## Citation

> US National Institutes of Health, RePORTER application 9982860, High Throughput Analysis Laboratory Shared Resource (5P30CA060553-26). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9982860. Licensed CC0.

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