# Physiology Core

> **NIH NIH P30** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2020 · $145,184

## Abstract

Abstract
The Physiology Core provides a series of graded in vitro technologies for studying the function and regulation
of transport and other membrane resident proteins with progressive degrees of complexity from single
molecules to model systems to native epithelia. To gain understanding of biological processes that mediate
kidney function in health and disease states, in light of the phenotypic diversity and functional complexity of this
organ, the use of strategies that allow specific segment/cell/protein to be studied in isolation under defined
conditions is required. The overall goal of this core is to elucidate at a molecular and cellular level the function
and regulation of key proteins involved in kidney health (including development) and disease. To accomplish
this goal, the Core will provide investigators with: a) microdissected tubules for analysis of RNA expression
and/or abundance, protein expression, immunolocalization, and enzyme/transporter microassays, b) functional
fluorescence assays of channel/transporter function in individually identified cells in isolated tubules
microperfused in vitro, c) measurements of transepithelial ion/solute fluxes across isolated tubules
microperfused in vitro, d) electrophysiological assays for functional analysis of transport proteins in
heterologous expression systems, either wild-type or mutated, alone or in combination with putative regulatory
proteins, e) analyses of transcellular and paracellular transport in model and native epithelia in Ussing
chambers, f) technologies for analysis of post-translational modification of proteins including phosphorylation,
ubiquitination, palmitoylation and glycosylation, and g) technologies to study composition, dynamics, and
regulatory mechanisms through the assessment of transcript abundance in isolated single cells. A central
function of the Core is to provide instruction in all the techniques performed by the Core and the use of the
Core equipment. The Physiology Core will interact synergistically with the other Cores to: a) extend the studies
conducted in either heterologous expression systems or isolated tubules to animals models, b) identify
potential therapeutic compounds that target transporters and associated regulatory pathways, and c) perform
detailed and quantitative analysis using imaging technologies of the expression of transport and regulatory
proteins in heterologous expression systems, model and native epithelia, and isolated tubules.

## Key facts

- **NIH application ID:** 9983064
- **Project number:** 5P30DK079307-13
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** Marcelo Daniel Carattino
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $145,184
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9983064

## Citation

> US National Institutes of Health, RePORTER application 9983064, Physiology Core (5P30DK079307-13). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9983064. Licensed CC0.

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