# Caspases in Inflammatory Cell Death Networks

> **NIH NIH R01** · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · 2020 · $523,976

## Abstract

PROJECT SUMMARY
Chronic inflammation is a major cause of cancer, diabetes, and neurodegeneration. Inflammation is a complex
topic, with both positive and negative effects on disease and therapy, and if we are to understand the
pathological results and therapeutic benefits we need to better understand the mechanisms of where
inflammation originates. The big picture in which this proposal is set is to understand the mechanisms of
inflammation as they relate to regulated cell death. This application seeks to substantially advance current
approaches, concepts, and technology to delve deeply into the inflammatory consequences of cell death and
the connections between the cell death mechanisms. We combine biochemistry, cell biology, genome editing,
non-animal models of inflammation, protein engineering, and chemical biology to explore linked pro- and anti-
inflammatory cell death mechanisms. We propose that a robust and quantitative approach to defining
inflammation is an important innovative quality of this proposal. The main goals of this proposal are to 1)
compute the difference in inflammatory mediators released as a result of apoptosis, pyroptosis and necroptosis
in macrophages, 2) define the mechanism of cytokine release from cells undergoing inflammatory cell death,
and 3) understand the activation mechanisms of caspases in pyroptosis, and synthesize highly selective
fluorescent probes to allow for direct visualization of specific caspase activation.

## Key facts

- **NIH application ID:** 9983684
- **Project number:** 5R01GM099040-08
- **Recipient organization:** SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
- **Principal Investigator:** Guy S. Salvesen
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $523,976
- **Award type:** 5
- **Project period:** 2012-05-01 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9983684

## Citation

> US National Institutes of Health, RePORTER application 9983684, Caspases in Inflammatory Cell Death Networks (5R01GM099040-08). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9983684. Licensed CC0.

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