# Mechanical regulation of actin binding proteins

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA BERKELEY · 2020 · $314,000

## Abstract

Project Summary
PI: Daniel Fletcher, Ph.D.
NIH R01 application, PA-18-484
Project Title: Mechanical regulation of acting binding proteins
Project Period: 07/01/2019 – 06/30/2024
The actin cytoskeleton consists of spatially organized structures of filaments that are architecturally distinct and
formed from over 300 different actin-binding proteins. One of the key roles of actin networks in cells is to
generate, transmit, and resist physical forces, but whether the forces carried by actin filaments influence the
binding of actin-binding proteins is not well understood. This proposal will investigate the ability of tandem
calponin-homology domains (CH1-CH2) to bind actin filaments in a tension-dependent manner, and it will explore
the functional impact of tension-dependent binding through a set of in vitro experiments measuring the
mechanical properties of actin gels with tension-dependent crosslinkers and live cells experiments quantifying
the effect of tension-dependent signaling and cytoskeletal organization on crawling motility. The outcome of this
work will be a new understanding of how force can organize actin networks and influence the function of both
healthy and diseased cells.

## Key facts

- **NIH application ID:** 9983733
- **Project number:** 5R01GM134137-02
- **Recipient organization:** UNIVERSITY OF CALIFORNIA BERKELEY
- **Principal Investigator:** DANIEL A FLETCHER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $314,000
- **Award type:** 5
- **Project period:** 2019-07-25 → 2023-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9983733

## Citation

> US National Institutes of Health, RePORTER application 9983733, Mechanical regulation of actin binding proteins (5R01GM134137-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9983733. Licensed CC0.

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