# CORE 2: Technology Core

> **NIH NIH U19** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2020 · $419,748

## Abstract

CORE 2: TECHNOLOGY CORE
SUMMARY
The objective of the Technology Core is to provide cutting-edge and innovative technologies for the functional
characterization of the genome and proteome in a reliable, reproducible, and cost-efficient manner. It will
support ​Project 1 ​and Project 2 by facilitating proteomics, genetic interaction, and structural protein
characterization experiments. In particular, the Technology Core will perform mass spectrometry
characterization of host-pathogen protein-protein interactions (PPIs) and infection induced signaling network
rewiring, CRISPR gene editing, and structural characterization by x-ray crystallography, cryo-electron
microscopy (cryo-EM), and cross-link mass spectrometry (XL-MS). This core will be comprised of three well
established facilities: the Thermo Fisher Scientific Proteomics Facility for Disease Target Discovery located at
the J. David Gladstone Institutes, the UCSF Molecular Structures Group core facility located at the Mission Bay
Campus of UCSF, and the Keck Advanced Microscopy Laboratory located at the Mission Bay Campus of
UCSF. Additionally, we propose to create a new Functional Genomics Core (FGC) that leverages the Marson
and Krogan lab’s ​expertise in genetic interaction mapping and genome engineering.
The Technology Core will be led by pioneers in their respective fields, and who will employ state-of-the-art
techniques, many of which were developed or optimized in their respective labs. The technologies described
here will be used extensively by ​Project 1 ​and Project 2 investigators to accomplish the individual aims of this
proposal, and will also serve as a resource to the wider scientific community by disseminating new assays and
protocols.

## Key facts

- **NIH application ID:** 9984275
- **Project number:** 5U19AI135990-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Alexander Marson
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $419,748
- **Award type:** 5
- **Project period:** 2018-08-17 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9984275

## Citation

> US National Institutes of Health, RePORTER application 9984275, CORE 2: Technology Core (5U19AI135990-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9984275. Licensed CC0.

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