# UC Irvine AD Translational Center for Disease Model Resources-Supplement to Purchase epMotion

> **NIH NIH U54** · UNIVERSITY OF CALIFORNIA-IRVINE · 2020 · $143,385

## Abstract

Project Summary/Abstract
A key goal of the UCI Model-AD Disease Model Development and Phenotyping Project (DMDPP) is to
characterize longitudinal gene expression levels of the new rodent models of sporadic AD being generated by
the consortium during aging. This involves, at a minimum, the building of 60 RNA-seq libraries for every mouse
model, plus early pilot RNA Seq to prioritize the models to be deep sequenced. We estimate close to 1500
libraries to be generated in the next 3 years. While the protocols for building these libraries are standardized
for technicians to carry out as samples become available, subtle manual variations in these procedures can be
a source of technical batch effects that can confound the interpretation of the resulting data. In order to
minimize the amount of technical variation introduced by human involvement, we are requesting funds to
purchase an Eppendorf epMotion 5075tC system in order to automate the reliable, reproducible building of
RNA-seq libraries produced by UCI Model-AD. The resulting decrease in technical variation will increase the
value of the resulting RNA-seq libraries for the characterization of the changes of gene expression during
aging in the UCI model-AD rodent models. This reliability enhances the translational outcome when comparing
with human data at multiple stages.

## Key facts

- **NIH application ID:** 9985454
- **Project number:** 3U54AG054349-03S6
- **Recipient organization:** UNIVERSITY OF CALIFORNIA-IRVINE
- **Principal Investigator:** FRANK M LAFERLA
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $143,385
- **Award type:** 3
- **Project period:** 2017-09-15 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9985454

## Citation

> US National Institutes of Health, RePORTER application 9985454, UC Irvine AD Translational Center for Disease Model Resources-Supplement to Purchase epMotion (3U54AG054349-03S6). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9985454. Licensed CC0.

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