# Animal and Microsurgery Core (Core B)

> **NIH NIH P01** · UNIVERSITY OF CHICAGO · 2020 · $398,198

## Abstract

CORE SUMMARY
The purpose of the Animal and Microsurgery Core (Core B) is to establish a focused facility that provides
investigators in each of the two projects with sufficient breeding of animals with specified genotypes and with
standardized cardiac or skin transplantation and tolerance induction procedures for the proposed experiments.
Core B will provide a key service role that will enable the Principal Investigators and their staff to focus on
experimental and scientific efforts. Centralized breeding and screening of the progeny will ensure that animals
of required genotypes are available for experiments. Transplantation performed by a centralized source will
standardize the procedures, minimize surgically imposed disparities in the grafts used by the two projects and
allow some long-term acceptors generated for Project 1 (studying novel T cell mechanisms in robust tolerance)
to be used for Project 2 (studying the maintenance of tolerance) to minimize experimental costs. In addition,
the purchase of supplies, such as sutures, anesthetics and immunosuppressants will be done in bulk for both
projects to reduce cost. The specific tasks of Core B will be to: a) breed and genotype animals to be used for
both projects; b) transplant heart allografts as directed by the project leaders and their personnel; c) prepare
and inject donor splenocytes and administer indicated immunosuppressive therapies; d) monitor the beating of
the transplanted hearts, the health of the transplant recipients, and record times of rejection; e) sacrifice
transplanted animals as directed by the Principal Investigators and their personnel, and collect organs for
histology, as well as to distribute to other project personnel for phenotypic and functional analysis of immune
cells.

## Key facts

- **NIH application ID:** 9985705
- **Project number:** 5P01AI097113-09
- **Recipient organization:** UNIVERSITY OF CHICAGO
- **Principal Investigator:** Anita S Chong
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $398,198
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9985705

## Citation

> US National Institutes of Health, RePORTER application 9985705, Animal and Microsurgery Core (Core B) (5P01AI097113-09). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9985705. Licensed CC0.

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