# Systematic dissection of gut cellular circuits [at single cell resolution]

> **NIH NIH RC2** · BROAD INSTITUTE, INC. · 2020 · $1,795,970

## Abstract

ABSTRACT
The gut serves as a nexus that connects and integrates organ systems throughout the body, with far-reaching
consequences in health and disease. The biological function of the gut as an organ is the result of complex
interactions between dynamic and heterogeneous cell types. Any pathological insult to the gut, such as barrier
breach or infection, must be met with a response that is appropriate and proportional to the insult, such that
homeostasis is re-established. In this application, we propose novel single-cell genomic approaches to
decipher heterogeneous cellular responses to inflammatory challenge. Through a series of functional genetics
studies, we aim to elucidate the molecular basis of mucosal immunity in the context of pathological
inflammation. Towards this end, we propose the following specific aims:
Aim 1. Comprehensively define all cell lineages and dynamic functional states in intestinal inflammation.
Aim 2. Delineate intercellular communication networks that coordinate intestinal inflammation.
Aim 3. Identify disease modifier pathways in human biopsies and integrate with intercellular communication
networks.

## Key facts

- **NIH application ID:** 9985804
- **Project number:** 5RC2DK114784-04
- **Recipient organization:** BROAD INSTITUTE, INC.
- **Principal Investigator:** Orr Ashenberg
- **Activity code:** RC2 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $1,795,970
- **Award type:** 5
- **Project period:** 2017-08-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9985804

## Citation

> US National Institutes of Health, RePORTER application 9985804, Systematic dissection of gut cellular circuits [at single cell resolution] (5RC2DK114784-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9985804. Licensed CC0.

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