# Functional genomics resources for the Drosophila - TR&D1

> **NIH NIH P41** · HARVARD MEDICAL SCHOOL · 2020 · $326,732

## Abstract

PROJECT SUMMARY – TR&D1 
In this project, we focus on development of functional genomics technologies for use in Drosophila cells and/or 
in vivo. Studies in cells provide a platform for large-scale gene function discovery using loss-of-function (e.g. 
CRIPSR knockout and CRISPR interference) and gain-of-function (e.g. CRISPR activation) approaches, and 
serves as an efficient platform to test technologies prior to their applications in vivo. Our plans balance 
relatively low-risk refinement of Drosophila pooled screen approaches with higher-risk but potentially highly 
impactful development of new CRISPR/Cas libraries and applications, e.g. using variant Cas9 forms with 
alternative protospacer-adjacent motifs, CRISPR inhibition (CRISPRi), and/or CRISPR-based targeting of 
RNA. A set of collaborators with interest in using screens and in vivo technologies to study cancer, rare 
diseases, cell biology, and other topics will help test and improve the technologies, and for screens in 
particular, the accumulation of data from multiple projects will help improve reagent design, data analysis, off- 
target prediction, etc. Altogether, this project will establish CRISPR pooled-format screens as a robust platform 
for analysis of conserved gene function, as well as result in new and improved cell and in vivo approaches, 
contributing to effective and efficient use of Drosophila to study health and disease.

## Key facts

- **NIH application ID:** 9985995
- **Project number:** 5P41GM132087-02
- **Recipient organization:** HARVARD MEDICAL SCHOOL
- **Principal Investigator:** STEPHANIE E MOHR
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $326,732
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9985995

## Citation

> US National Institutes of Health, RePORTER application 9985995, Functional genomics resources for the Drosophila - TR&D1 (5P41GM132087-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9985995. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*