# Functional genomics resources for the Drosophila - TR&D2

> **NIH NIH P41** · HARVARD MEDICAL SCHOOL · 2020 · $326,732

## Abstract

PROJECT SUMMARY – TR&D2 
Drosophila serves as a model not only of human biology but also of the biology of other dipterans, including 
mosquito vectors of disease, and provides an instructive example of technologies that can be applied to direct 
study of other insects. In this project, we focus on development of functional genomics and proteomics 
resources for the study of mosquito vectors of disease, with a particular focus on development of CRISPR 
pooled screens for Aedes mosquitoes, which are vectors of viral pathogens including Zika, Dengue, and 
Chikungunya viruses, and for Anopheles mosquitoes, the vector for malaria. We will additionally develop a 
pipeline for isolation of robust protein binders, i.e. nanobodies, for use in visualization and in vivo functional 
blocking, based on a yeast nanobody display platform. A set of collaborators with established assays in 
mosquitoes will help us to test and improve the screening and nanobody technologies. Altogether, this project 
will provide much-needed reagents, including a platform for genome-wide screening, for direct study of 
pathogen entry and other aspects of mosquito biology relevant to infectious diseases and mosquito population 
control.

## Key facts

- **NIH application ID:** 9985996
- **Project number:** 5P41GM132087-02
- **Recipient organization:** HARVARD MEDICAL SCHOOL
- **Principal Investigator:** STEPHANIE E MOHR
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $326,732
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9985996

## Citation

> US National Institutes of Health, RePORTER application 9985996, Functional genomics resources for the Drosophila - TR&D2 (5P41GM132087-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9985996. Licensed CC0.

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