# Single Cell Phenotyping Core

> **NIH NIH P30** · CINCINNATI CHILDRENS HOSP MED CTR · 2020 · $89,936

## Abstract

Several cell types are involved in inflammatory processes in rheumatic diseases. Understanding the
pathobiology of these diseases often requires dissecting out contributions of specific cell subpopulations.
The SCPC (Single Cell Phenotyping Core) will provide technical expertise, assistance and equipment at a
reasonable cost to investigators in the Research Base of the Cincinnati Rheumatic Disease Core Center
(CRDCC) whose studies require single cell analysis. The SCPC will provide training in the proper use and
interpretation of a number of immunophenotyping methodologies, including flow cytometry and single cell
gene expression. Expertise and assistance will be provided by Core personnel in the following areas:
· Cell sorting
· Cell surface phenotyping
· Intracellular cytokine detection
· Gene expression reporter analysis
· Cell proliferation analysis
· Cell cycle and DNA content analysis
· Apoptosis analysis
· Intracellular calcium mobilization analysis
· Tetramer staining of antigen-specific T cells
· RNA detection by flow cytometry
· Cell signalling analysis
· CRISPR genome-edited cell identification and sorting
· Imaging cytometry
· Single cell manipulation and gene expression analysis
In addition, the SCPC will facilitate acquisition, analysis and storage of immunophenotyping data, maintain
standards and quality control for immunophenotyping procedures, and assist in the application and
development of new techniques.

## Key facts

- **NIH application ID:** 9986691
- **Project number:** 5P30AR070549-05
- **Recipient organization:** CINCINNATI CHILDRENS HOSP MED CTR
- **Principal Investigator:** SHERRY L THORNTON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $89,936
- **Award type:** 5
- **Project period:** 2016-08-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9986691

## Citation

> US National Institutes of Health, RePORTER application 9986691, Single Cell Phenotyping Core (5P30AR070549-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9986691. Licensed CC0.

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