# Core 2: Cell and Whole Animal Genome Engineering

> **NIH NIH U54** · UNIVERSITY OF MINNESOTA · 2020 · $40,293

## Abstract

Abstract
Core 2
Core 2, Cell and Whole Animal Genetic Engineering, will provide both projects with genetically engineered cell
lines and mice for analyzing cell and/or tumor behavior under a variety of conditions. The core brings together
expertise from two highly trained mouse and somatic cell geneticists, Dr. David Largaespada and Dr. Anindya
Bagchi, both with expertise in modeling cancer in mice and with a wide array of cells. Thus, this core will
generate two specific resources for both projects #1 and #2. The first is cohorts of mice that reliably develop
pancreatic, glioma or medulloblastoma tumors due to the inheritance of germline transgenes, mutant alleles in
their endogenous locus, or due to transposon-mediated gene delivery to the brain. Likewise, Core 2 will
support studies perturbing the genetic events examined in each project. Additional models will be developed as
needed. The models will be provided to project #1 and #2 scientists in age-matched cohorts and of both
genders. These models are based both on the published literature well known to Dr. Largaespada, Dr. Bagchi
and Dr. Provenzano, as well as technologies developed in the Largaespada lab. Specifically, these will be
models of glioma and medulloblastoma initiated in mice by transposon delivery of shRNA and cDNAs. A
second resource is to provide genetically altered human cancer cell lines for project #1 and #2. These cell lines
will harbor loss of function mutations, or specific single nucleotide variants (SNV), in genes of interest.
They will be produced using TAL endonuclease or CRISPR/Cas9 gene targeting, without or with
oligonucleotide substrates, to create “knockout” cell lines due to imprecise non-homologous end joining (NHEJ)
in the former case, or SNVs via homology directed repair (HDR) in the latter case.

## Key facts

- **NIH application ID:** 9987341
- **Project number:** 5U54CA210190-05
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **Principal Investigator:** DAVID ANDREW LARGAESPADA
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $40,293
- **Award type:** 5
- **Project period:** 2016-08-17 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9987341

## Citation

> US National Institutes of Health, RePORTER application 9987341, Core 2: Cell and Whole Animal Genome Engineering (5U54CA210190-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9987341. Licensed CC0.

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