# Animal/Virology Core

> **NIH NIH P01** · UNIVERSITY OF IOWA · 2020 · $296,783

## Abstract

ABSTRACT
The Animal/Virology Core Laboratory is directed by a senior, experienced postdoctoral fellow,
Dr. Rudragouda Channappanavar, under the guidance of the Core Director, Dr. Perlman. The
Core will be primarily based in a University of Iowa BSL3 laboratory that is equipped for tissue
culture and animal work. Personnel working in the Core are experienced in virological and cell
culture techniques and in handling, infecting and analyzing mice. The Animal/Virology Core has
several functions that are critical for success of this PO1 grant. 1. It will maintain colonies of
hDPP4-knock-in mice. 2. It will propagate and titer nonrecombinant and recombinant mouse-
adapted and human strains of MERS-CoV and SARS-CoV and will infect mice with these
viruses. 3. It will monitor mice for clinical disease and weight loss and will harvest tissue. 4. The
Core will prepare samples for histological and immunohistochemical analysis and will prepare
RNA and protein from infected tissue. 5.The Core will analyze mice for virus-specific antibody
and T cell responses. 6. The Core will develop recombinant MERS-CoV and SARS-CoV using
BAC DNA clones prepared by the individual projects. The Core will also be responsible for
ensuring that protocols for working with animals and for working under BSL3 laboratory
conditions are up-to-date. While the Core will teach members of the Project methods important
for analysis of mice under BSL3 conditions, it will primarily be responsible for performing most of
these analyses. The Core will provide reagents and perform common assays efficiently, thereby
standardizing results and enhancing synergistic interactions. Additionally, all projects will
interact with the Core, thereby facilitating interactions between members of the PPG. By
providing these services, the Core will allow Program investigators to focus on issues related to
MERS and SARS pathogenesis and to the development of anti-viral therapies and vaccines.

## Key facts

- **NIH application ID:** 9988154
- **Project number:** 5P01AI060699-14
- **Recipient organization:** UNIVERSITY OF IOWA
- **Principal Investigator:** Stanley Perlman
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $296,783
- **Award type:** 5
- **Project period:** 2004-07-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9988154

## Citation

> US National Institutes of Health, RePORTER application 9988154, Animal/Virology Core (5P01AI060699-14). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9988154. Licensed CC0.

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