# Enable ultra-high throughput, ultra-sensitive and more quantitative broad proteomics measurements from much smaller samples

> **NIH NIH P41** · BATTELLE PACIFIC NORTHWEST LABORATORIES · 2020 · $519,803

## Abstract

Project Summary – TR&D 2
The Resource has the overall goal of broadly impacting biomedical research by providing the abilities to: obtain
high quality proteomics data from much smaller samples, produce more quantitative and comprehensive
measurements, generate improved and more extensive information on low abundance components, distinguish
presently problematic peptide isomers, and enable the study of much larger sample sets than presently
practical by providing increases in measurement sensitivity and throughput. The technology efforts of TR&D 2
will enable these capabilities by providing high resolution separations and ion manipulations with ion mobility-
mass spectrometry that result in proteomics measurements with much greater sensitivity and throughput,
which in conjunction with the `front-end' developments under TR&D 1, will provide the basis for broadly
effective applications of proteomics from much smaller samples. TR&D 2 will develop new platforms based on
our novel Structures for Lossless Ion Manipulations (SLIM) technology. These platforms will provide ultra-fast
and ultra-high resolution ion mobility (IM) separations that will complement the distinctive advantages
provided by both fast time-of flight MS and ultra-high mass accuracy and resolution FTMS (specifically
Orbitrap-based MS) platforms. This work will benefit from the inherently fast, robust, and highly reproducible
nature of ultra-high resolution IM separations, as well as the distinctive peptide/protein size/shape
information (collision cross sections) provided by IM. In combination with the bioinformatics developments of
TR&D 3, TR&D 2 will enable improved quantification and proteome coverage in conjunction with high
throughput measurements. Overall, TR&D 2 will enable: (1) much more extensive proteomic information from
extremely small samples (e.g. from Fluorescence-activated cell sorting and laser capture microdissection) when
applied in conjunction with the developments of TR&D 1; (2) large gains in proteomic throughput
(measurement speeds) based upon fast ultrahigh-resolution IM separations and related MS-based advances;
(3) more quantitative and higher data quality measurements that effectively provide a convergence of the
attractive properties of global and targeted MS-based proteomic measurements; and (4) broader proteome
measurement coverage that also addresses important `blind spots' of current technologies, such as presently
indistinguishable PTMs and other peptide isomers. These efforts will build upon previous Resource
developments and will be facilitated by key computational and bioinformatics tool developments under
TR&D 3. In combination, these efforts will provide a basis for rapid implementation and initial evaluation of
the new capabilities in a set of challenging biomedical projects, as well as their effective dissemination to the
research community, including seeding the technology in several outside laboratories and facilitating their
initial commercial...

## Key facts

- **NIH application ID:** 9988428
- **Project number:** 5P41GM103493-18
- **Recipient organization:** BATTELLE PACIFIC NORTHWEST LABORATORIES
- **Principal Investigator:** RICHARD D SMITH
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $519,803
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9988428

## Citation

> US National Institutes of Health, RePORTER application 9988428, Enable ultra-high throughput, ultra-sensitive and more quantitative broad proteomics measurements from much smaller samples (5P41GM103493-18). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9988428. Licensed CC0.

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