# TR&D 1 Isobaric Mass Tags for Ultra-Plexed Protein Quantification p. 398

> **NIH NIH P41** · UNIVERSITY OF WISCONSIN-MADISON · 2020 · $218,326

## Abstract

Project Summary 
The continued development of protein quantification technologies is key to modern biology and medicine. 
Indeed, the ability to parallel process samples during quantitative analysis (multiplex) is essential for many of the 
proposed DBP applications. Sample multiplexing via chemical labeling offers numerous key advantages. First, 
parallel processing of samples in a single mass spectrometric analysis reduces the amount of material required 
from any one sample; this is particularly relevant for tissue-based work. Second, multiplexing greatly reduces 
sample preparation and instrument analysis time requirements, which is essential for the expanded application 
of proteomic analysis to translational medicine. Third, highly plexed analyses facilitate collection of biological 
replicate data, an obvious requisite for the field's advancement. Fourth, the ability to combine samples into a 
single analysis permits fewer MS experiments, which in turn allows for greater data overlap across all conditions. 
Finally, chemical labeling is compatible with biological tissues and fluids. We conclude the ability to analyze 
biological fluids and tissues is vital for the application of proteomics to translational medicine. In this TR&D 
project we aim to expand chemical labeling to an unprecedented 30-plex analysis, with the possibility to go much 
higher, by synthesis of new neutron-encoded chemical tags. Further, we will develop new technologies to boost 
MS/MS sampling overlap, since an MS/MS scan and identification is required to obtain quantitative data from 
isobaric tagging.

## Key facts

- **NIH application ID:** 9988437
- **Project number:** 5P41GM108538-05
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **Principal Investigator:** LINGJUN LI
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $218,326
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9988437

## Citation

> US National Institutes of Health, RePORTER application 9988437, TR&D 1 Isobaric Mass Tags for Ultra-Plexed Protein Quantification p. 398 (5P41GM108538-05). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9988437. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*