# Mutation Generation and Detection Core (Core D)

> **NIH NIH U54** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 2020 · $50,937

## Abstract

PROJECT SUMMARY
The Mutation Generation and Detection Core (MGD) specializes in providing reagents for targeted genome
editing. Services include knocking out specific genes, creating genes with limited or altered expression
patterns, and tagging genes to express specific proteins sequence tags. Investigators can order custom
TALEN and Crispr-Cas9 DNA nucleases to induce targeted mutations in a genomic region of interest. These
targeted nucleases are a cutting edge technology for performing reverse genetic studies in multiple model
systems, including, but not limited to, Zebrafish, Drosophila, C. elegans, mice and mammalian tissue
culture. The Core continues to develop new technologies to offer investigators the most efficient reagents and
methods possible. Through the Center for Iron and Heme Disorders (CIHD), investigators can be trained in the
design, construction and delivery of the genome editing reagents. Additionally, the Core will assist users in
designing strategies to analyze correct targeting and ensure that there are no off-target events. The staff will
also offer CIHD-sponsored workshops/training sessions for junior investigators. This technology will also be
made available to other national NIDDK-sponsored research programs. The primary goal of the MGD Core is
to expand the use of these genome-editing technologies to NIH-funded investigators.

## Key facts

- **NIH application ID:** 9990557
- **Project number:** 5U54DK110858-05
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **Principal Investigator:** TIMOTHY John DAHLEM
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $50,937
- **Award type:** 5
- **Project period:** 2016-08-05 → 2021-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9990557

## Citation

> US National Institutes of Health, RePORTER application 9990557, Mutation Generation and Detection Core (Core D) (5U54DK110858-05). Retrieved via AI Analytics 2026-06-02 from https://api.ai-analytics.org/grant/nih/9990557. Licensed CC0.

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