# Live Imaging Core

> **NIH NIH P01** · UNIVERSITY OF OREGON · 2020 · $180,999

## Abstract

PROJECT SUMMARY/ABSTRACT (LIVE IMAGING CORE)
The Live Imaging Core provides training in the use of, supervised access to, and maintenance of state-of-the-
art imaging equipment, as well as the continued development of instrumentation and analysis methods. The
three major equipment components of this Core, custom-built light sheet fluorescence microscopes,
instrumentation for high throughput sample handling for light sheet microscopy, and tools for low-
magnification fluorescence imaging are central to achieving the aims of the Research Projects. The technical
expertise of this Core will ensure that all Project research is carried out on instrumentation that pushes the
current boundaries for visualization of microbes within the zebrafish intestine. We have already demonstrated
the ability to visualize larval zebrafish intestinal microbes with single-bacterium resolution, watching bacterial
growth and competition and extracting quantitative information from the resulting images; these capabilities
are requisite for our proposed Project experiments. Because our instrumentation is custom designed and built,
it allows maximum flexibility for experimenters, including use of multiple fluorescent probes, and integration
of custom-written software to control various hardware in ways tailored to the proposed experiments. In
addition, image analysis code is also achieved by custom-developed software that makes use of semi-
automated methods and machine-learning-based classification objects; ongoing work aims to further optimize
these approaches. Our custom developed high throughput methods address the challenge of imaging large
numbers of specimens and we are developing low-magnification “macroscope” methods that employ simple
yet versatile cameras and filters to rapidly assess abundances of fluorescent organisms over wide fields of
view. This Core will continue to design and refine instrumentation. At the same time Core personnel will train
Program Project research personnel in use of this instrumentation, and work with them to design experiments
that maximize efficiency and productivity. Achieving the aims of this Core will significantly enhance the
ability to carry out experiments described in the Research Projects.

## Key facts

- **NIH application ID:** 9990821
- **Project number:** 5P01GM125576-03
- **Recipient organization:** UNIVERSITY OF OREGON
- **Principal Investigator:** Raghuveer Parthasarathy
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $180,999
- **Award type:** 5
- **Project period:** 2018-08-06 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9990821

## Citation

> US National Institutes of Health, RePORTER application 9990821, Live Imaging Core (5P01GM125576-03). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9990821. Licensed CC0.

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