# Core-001: Histology, Biochemistry and Molecular Imaging (HBMI) Core

> **NIH NIH P30** · UNIVERSITY OF ROCHESTER · 2020 · $209,594

## Abstract

The Histology, Biochemistry, and Molecular Imaging (HBMI) Core will provide musculoskeletal histopathology, 
histomorphometry, and biochemistry as well as cellular and molecular imaging and analysis services and state- 
of-the-art resources and training to support the collaborative clinical and basic science components of the 
Center for Musculoskeletal Research (CMSR), all under a single administrative structure led by Drs. Brendan 
F. Boyce and Jennifer H. Jonason. The HBMI Core is divided into four research and service Sub-Cores: 1) 
Histology, Immunohistochemistry (IHC), and In-Situ Hybridization (ISH); 2) Microscopy, Histomorphometry, 
and Imaging; 3) Biochemistry, Cellular, and Molecular Biology; and 4) Small Animal Surgery. Several skilled 
New Investigators and laboratory technicians will provide training in techniques and the use of sophisticated 
instrumentation within each Sub-Core. The expansion and integration of services within the HBMI Core will 
further increase productivity, enhance the efficiency of the Core and enable the translation between histologic 
and histomorphometric data and the underlying cellular and molecular mechanisms in musculoskeletal 
diseases. In addition, the HBMI Core will develop novel technologies and approaches to generate and analyze 
histologic, histomorphometric, biochemical, cellular, and molecular data in the musculoskeletal sciences, which 
will accelerate the pace of research for all funded projects within the CMSR. The overall Specific Aims of the 
Core are to: 1) provide cost-efficient and high quality histology, IHC, IF, ISH, imaging, and histomorphometry 
services to URRBCMBM scientists and their collaborators; 2) provide the above services at no cost to our New 
Investigators, who in turn will assist the Core by teaching their unique and varied skills to students and 
postdoctoral fellows within the CMSR; 3) provide URRBCMBM faculty and their collaborators access to state- 
of-the-art molecular resources, surgical equipment and suites within the HBMI Core and help New 
Investigators to train staff, students, and postdoctoral fellows to perform biochemical assays and small animal 
surgeries; and 4) innovate HBMI musculoskeletal basic and clinical research by continuing to: a) develop 
digital whole slide imaging WSI technology to generate novel algorithms for automated, quantitative analysis of 
standard and novel parameters of musculoskeletal tissue morphology and of protein and gene expression by 
IHC, IF or ISH in these tissues using Visiopharm's image analysis system and to validate these parameters 
using the OsteoMetrics, Inc. system; b) develop protocols that will enable us to quickly prepare sections of 
fresh samples of human tissue for isolation of high quality nucleic acids using laser capture microdissection 
(LCM) to support the AMP (Anolik, PI) and other grants that aim to assess gene expression levels in individual 
or groups of cells in diseased tissues; and c) create tissue mic...

## Key facts

- **NIH application ID:** 9991749
- **Project number:** 5P30AR069655-05
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **Principal Investigator:** Brendan F Boyce
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $209,594
- **Award type:** 5
- **Project period:** — → 2021-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9991749

## Citation

> US National Institutes of Health, RePORTER application 9991749, Core-001: Histology, Biochemistry and Molecular Imaging (HBMI) Core (5P30AR069655-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9991749. Licensed CC0.

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