# The role of adaptor proteins in endosomal sorting during ultrafast endocytosis

> **NIH NIH F32** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 2020 · $64,926

## Abstract

Summary
Endocytosis is a process by which macromolecules, receptors, transporters, and
channels are recycled via internalization of the plasma membrane. Endocytosis at
synapses, called synaptic vesicle endocytosis, supports the rapid recovery of vesicles
during neurotransmission. Clathrin-mediated endocytosis occurs ~ 30 s after stimulation,
however using “flash-and-freeze” electron microscopy we have demonstrated
endocytosis at 30 – 300 ms after stimulation in both mouse hippocampal neurons as well
as C. elegans neuromuscular junctions. Further, the process is clathrin independent.
Ultrafast endocytosis generates a large endosome, which much be sorted into new
synaptic vesicles or targeted for degradation in the lysosomes. Sorting of the endosome
requires clathrin, but it is not clear which proteins act on the endosome to target these
vesicles. The clathrin binding adaptor proteins AP1, AP2, and AP3 have all been
implicated in synaptic function and targeting of membrane bound compartments. AP2 is
thought to regenerate synaptic vesicles, while AP1 and AP3 are thought to target vesicles
to the plasma membrane or the lysosome, respectively. The goal of this fellowship is to
determine the role of these adaptor proteins in endosomal sorting and regeneration of
synaptic vesicles during ultrafast endocytosis.

## Key facts

- **NIH application ID:** 9993007
- **Project number:** 1F32GM137601-01
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **Principal Investigator:** Kevin Jonathan Kruse
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $64,926
- **Award type:** 1
- **Project period:** 2020-09-01 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9993007

## Citation

> US National Institutes of Health, RePORTER application 9993007, The role of adaptor proteins in endosomal sorting during ultrafast endocytosis (1F32GM137601-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9993007. Licensed CC0.

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