# Application and Development of Antibody Tools

> **NIH NIH P50** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2020 · $323,828

## Abstract

The goal of Core 4 of the HARC Center is to generate recombinant antibody fragments (Fabs), single chain
Fvs (scFvs) and nanobodies that bind to different conformational states of HIV accessory and regulatory
proteins to enable and accelerate activities in all Projects and Cores. We will generate multiple Fabs to
stabilize protein complexes, to serve as fiducial markers in single particle cryo-EM studies and to act as
binding partners to accelerate crystallization studies. Information gained from Fab studies including binding
epitopes, structural data, and the pharmacologic effect of scFvs or nanobodies will be inputs for molecular
modeling to provide insights into the mechanism of HIV-host interactions. The Craik lab has a successful
record of generating recombinant Fabs to a variety of challenging targets. For example, together with Yifan
Cheng, we have demonstrated the utility of Fabs as fiducial markers for single particle cryo-EM studies of
proteins and with John Gross we have probed the activity of the CRL5-Vif-CBF E3 ligase in vitro and in cells
using Fabs and scFvs, which are described as examples. Given availability of reagent quality and quantity of
proteins from Projects 1-7 that include Vif, Vpu, Nef, Tat, Rev and APOBEC3s, and other targets from
additional collaborators. Identification of Fabs will enable similar studies to be performed in conjunction with the
individual Projects and Cores 3, 5 and 6 to determine structures and functions of the protein complexes.

## Key facts

- **NIH application ID:** 9993237
- **Project number:** 5P50AI150476-14
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Charles Scott Craik
- **Activity code:** P50 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $323,828
- **Award type:** 5
- **Project period:** 2007-08-27 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9993237

## Citation

> US National Institutes of Health, RePORTER application 9993237, Application and Development of Antibody Tools (5P50AI150476-14). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9993237. Licensed CC0.

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