# Structural Biology

> **NIH NIH P50** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 2020 · $168,979

## Abstract

ABSTRACT
Our Structural Biology Core provides capabilities for visualizing structures of macromolecules, subcellular
complexes, cells and tissues. X-ray crystallography (Aim 1) is essential for many of our projects, and has
already been used to determine high-resolution structures that yielded insights into HIV architecture and
maturation1-3, budding4-11, restriction12,13, immune recognition14-17, and novel therapeutics.18-23 Electron
crystallography (Aim 2) will provide structures from 2D crystals, e.g., studies of HIV-1 CA protein26, the CTD-
SP1 construct of Gag2 and the TRIM5α restriction factor.27,28 We are also developing methods for electron
diffraction from microcrystals29-31, which has considerable potential when large crystals are unavailable,
disordered, and/or have packing defects. Electron cryomicroscopy (cryo-EM) and single-particle image
reconstruction (Aim 3) are transforming structural biology32, and we have implemented state-of-the-art
technology, and previously determined structures that illuminate HIV budding33 and immune recognition.34,35
We have also implemented cutting-edge methods of EM tomography (Aim 4) and applied them to image
structures such as authentic viral cores24,36, cores bound by the innate restriction factor TRIM5α28, subcellular
structures localized by fluorescence microscopy37, and HIV within tissues.25

## Key facts

- **NIH application ID:** 9993250
- **Project number:** 5P50AI150464-14
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **Principal Investigator:** CHRISTOPHER P. HILL
- **Activity code:** P50 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $168,979
- **Award type:** 5
- **Project period:** 2007-08-27 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9993250

## Citation

> US National Institutes of Health, RePORTER application 9993250, Structural Biology (5P50AI150464-14). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9993250. Licensed CC0.

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