# Consortium for large-scale production and phenotyping of knockout mice (UM1)

> **NIH NIH UM1** · BAYLOR COLLEGE OF MEDICINE · 2020 · $5,851,996

## Abstract

Consortium for large-scale production and phenotyping of knockout mice (UM1)
ABSTRACT:
The long-term goal of the International Knockout Mouse Consortium (IKMC) is to develop a resource of
targeted mutations in mice for every protein-coding gene in the mammalian genome that the research
community can use to elucidate gene function in human biology and disease. We have formed a consortium of
two Institutes (Baylor College of Medicine and MRC Harwell). This application describes our plan to generate
null alleles for 1500 mouse genes using CRISPR/RGN genome editing technology and validate each line using
established QC procedures. We will cryopreserve all mutant strains and deliver germplasm to the MMRRC
repositories. These mutant alleles represent a gold standard resource of mutant alleles for the wider
community, and an important foundation for future research and translational studies using the mouse models
created. We will perform broad-based adult phenotyping on all mutant lines, and for the first time incorporate
an ageing component for a significant fraction of mutants, the latter involving an additional testing component
from 12 months onwards. We will also assess homozygous lethal and subviable lines in an embryonic
phenotyping pipeline, It is apparent that there is a relationship of mouse lethal (essential) and subviable genes
with human disease loci. All allele and phenotype data will be submitted in real time to the Data Coordination
Center, ensuring that all of the BasH data is disseminated to the wider biomedical scientific community. We will
continue our R&D program to introduce appropriate methodological and technological developments from
production to phenotyping. BasH will continue to pilot improvements in Cas9 RGN for the production of more
sophisticated alleles, and in parallel ensure that developments in cryopreservation approaches at Baylor and
Harwell are integrated into the production pipeline. We will also continue our major projects in improving and
enriching the adult and embryo phenotyping pipelines, focusing on areas such as metabolomics, behavioral
phenotyping and home cage monitoring, with the aim of providing more complex and longitudinal data. Baylor
College of Medicine and MRC Harwell have the established expertise, experience, and resources to efficiently
and cost-effectively meet this goal.

## Key facts

- **NIH application ID:** 9993556
- **Project number:** 5UM1HG006348-10
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **Principal Investigator:** Mary E Dickinson
- **Activity code:** UM1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $5,851,996
- **Award type:** 5
- **Project period:** 2011-09-28 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9993556

## Citation

> US National Institutes of Health, RePORTER application 9993556, Consortium for large-scale production and phenotyping of knockout mice (UM1) (5UM1HG006348-10). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9993556. Licensed CC0.

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