# CORE B - FSH Glycoform Production,  Purification, and Analysis

> **NIH NIH P01** · WICHITA STATE UNIVERSITY · 2020 · $370,077

## Abstract

Project Summary:
Core B: FSH Glycoform Production, Purification and Analysis
Core B provides critical and requisite reagents, analyses, and expertise to support all of the Projects in fulfilling
their Specific Aims. Specifically, Core B will continue to produce recombinant hFSH glycoforms. Procedures
have been established to produce milligram quantities of glycoforms from the conditioned medium of cultured
GH3 cells transfected with the human FSH α and ß subunit genes. In conjunction with Project 3, Core B will
generate mutant hFSH glycoforms, which mimic known recombinant hypoglycosylated hFSH glycoforms and
supply them to all 3 Projects. Core B will continue to work with Project 1 to generate monoclonal antibodies
that recognize specifically intact hFSH, hFSH α subunit, as well as individual glycoform ß subunits, for use in
glycoform isolation and detection. Core B will provide a variety of cells for use in Projects 1 and 2 to assess
glycoform receptor binding and signal transduction and glycoform impact upon cell differentiation. Cells to be
furnished include primary porcine granulosa cells, which naturally express the FSH receptor, KGN cells, a
human granulosa cell line that expresses the hFSH receptor, CHO cells that express the human FSH receptor,
and GH3 cells transfected with hFSH genes for use in the production of recombinant hFSH. Core B will
provide a variety of analyses for Projects 1, 2, and 3 including FSH, LH and TSH RIA, FSH and LH receptor
binding, E2ß and P4 RIA, and ELISAs for urinary FSH and ELISA development for monoclonal antibody
screening. Core B will assist Project 1 in the analysis of recombinant hFSH glycoform circulating half-life
following administration to FSHß null mice. Core B will assist Project 1 in the analysis of FSH glycoform
abundance change as it relates to the menstrual cycle and the impact of aging. In addition, the relationship
between glycoform abundance and infertility will be studied through the continued development of rapid and
direct methods to determine hFSH glycoform abundance in urine. The goal is to develop urinary assessment
of hFSH glycoforms diagnostic as a clinical diagnostic tool.

## Key facts

- **NIH application ID:** 9994166
- **Project number:** 5P01AG029531-09
- **Recipient organization:** WICHITA STATE UNIVERSITY
- **Principal Investigator:** JEFFREY V. MAY
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $370,077
- **Award type:** 5
- **Project period:** 2009-04-15 → 2022-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9994166

## Citation

> US National Institutes of Health, RePORTER application 9994166, CORE B - FSH Glycoform Production,  Purification, and Analysis (5P01AG029531-09). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9994166. Licensed CC0.

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