# Core B - The Clinical and Biomarkers Core

> **NIH NIH P01** · UNIVERSITY OF MINNESOTA · 2020 · $272,404

## Abstract

ABSTRACT 
The Clinical and Biomarkers Core is critical for the studies in this program project renewal proposal. The Core 
will recruit smokers to collect biological samples and will carry out analyses of tobacco constituent biomarkers. 
The biological samples collected by the Core will be used to evaluate and compare biomarkers of tobacco 
exposure across Japanese Americans, Whites, and Native Hawaiians in Projects 2, 3, and 4. Biomarkers to be 
analyzed by the Core include total nicotine equivalents (nicotine intake), 4-(methylnitrosamino)-1-(3-pyridyl)-1- 
butanol (NNAL) and its N- and O-glucuronides (exposure to tobacco-specific lung carcinogen NNK), 
phenanthrene tetraol and 3-hydroxy phenanthrene (exposure to and metabolism of the representative 
polycyclic aromatic hydrocarbon phenanthrene), mercapturic acids (exposure to acrolein, crotonaldehyde, and 
propylene oxide), urinary cadmium, as well as urinary biomarkers and DNA adducts derived from oxidative 
stress and inflammation. The measurements will be carried out on samples from a total of 6,084 subjects, with 
some of these samples being analyzed for only a subset of biomarkers. The samples from these subjects will 
either be procured from the existing bioresposity of the Multiethnic Cohort or obtained from smokers who are 
recruited by this Core. All assays, except for urinary cadmium, are based on stable-isotope dilution mass 
spectrometry and all have been analytically validated with respect to accuracy and precision. Personnel in this 
Core have extensive experience in clinical trials and quantitation of urinary tobacco biomarkers and DNA 
adducts, and were the first to develop many of the methods that will be used in this program project grant. The 
biological samples and biomarker results generated by this Core will be used by all projects.

## Key facts

- **NIH application ID:** 9994208
- **Project number:** 5P01CA138338-10
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **Principal Investigator:** DOROTHY K HATSUKAMI
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $272,404
- **Award type:** 5
- **Project period:** — → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9994208

## Citation

> US National Institutes of Health, RePORTER application 9994208, Core B - The Clinical and Biomarkers Core (5P01CA138338-10). Retrieved via AI Analytics 2026-06-01 from https://api.ai-analytics.org/grant/nih/9994208. Licensed CC0.

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