# Core B: The Cell Culture Core

> **NIH NIH P01** · NORTHWESTERN UNIVERSITY · 2020 · $218,261

## Abstract

Project Summary: Core B
The Cell Culture Core, designated as Core B, is a centralized facility that provides primary mouse embryonic
fibroblasts, endothelial and epithelial cells isolated from wild-type and genetically modified mice to each of the
five projects. Core B also maintains secondary lung cell lines for the PPG and will generate genome-edited
cells. Core B will use advanced genome-editing technology to produce cells expressing mEmerald-vimentin,
both under the control of its endogenous promoter, as well CRISPR/Cas9-based genome editing to produce
cells lacking vimentin protein. The Core personnel have extensive experience in the isolation of primary mouse
embryonic fibroblasts, tissue-specific fibroblasts, endothelial, and epithelial cells from mouse tissue (e.g. lung
and liver), as well as general cell culture techniques. Centralization of the cell culture facilities will ensure that
continuous supply of high-quality cells will be available to each of the five projects. The consolidated cell
culture facility provides an economical means of isolating and culturing primary and secondary cells. This
translates into reduced overall costs (i.e., personnel, reagents, and animals) and more importantly maintains
the quality of the cells used in research. The Core will also provide investigators several murine strains,
including vimentinfl/fl mice, which will allow investigators to examine cell-specific roles of genes or pathways
using mice expressing Cre recombinase or using tamoxifen-inducible systems. Many of the strains will be used
in more than one project, highlighting the synergy and economies of scale achievable by this Program Project.
These animals will be used to generate cells and tissues for investigations outlined by the project investigators.
In addition, Core B will conduct limited RNA analysis to assess the specific gene expression in vimentin
expressing and vimentin-null cells using the Northwestern University Next-Generation Sequencing (NGS) Core
Facility.
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## Key facts

- **NIH application ID:** 9994766
- **Project number:** 5P01GM096971-08
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **Principal Investigator:** KAREN M RIDGE
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $218,261
- **Award type:** 5
- **Project period:** 2011-06-15 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9994766

## Citation

> US National Institutes of Health, RePORTER application 9994766, Core B: The Cell Culture Core (5P01GM096971-08). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9994766. Licensed CC0.

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