# Mouse Congenics Core

> **NIH NIH P01** · CASE WESTERN RESERVE UNIVERSITY · 2020 · $237,750

## Abstract

PROJECT SUMMARY
The Mouse Congenics Core B will provide centralized breeding for use in each of the individual projects of
SAMP1/YitFc (SAMP), TNFARE, and control (AKR/J) mice, as well as SAMP and TNFARE congenic lines
that carry targeted genetic deletions of key mediators of intestinal innate immunity that are relevant to the
research strategies of each project. Maintaining these mouse breeding colonies and experimental animals in a
common centralized facility under uniform environmental conditions continues to be crucial to the success of this
Program. Centralization of mouse production also significantly reduces costs by eliminating redundant breeding
colonies or the need to purchase mice commercially, and ultimately reduces the numbers of mice required by
ensuring efficient utilization by each of the projects. A second aim is to provide centralized production of
genetically-mutated SAMP and TNFARE mice via marker-assisted breeding to significantly shorten the time
required to generate these congenic sub-strains. Transfer of induced mutations into an appropriate background
can be accomplished in 5 generations using this approach. The third aim of the core is to provide regular
monitoring and internal quality control of histopathological scoring of intestinal inflammation for PPG
experimental mice used in all three projects, a service that will be heavily used through the Histology/Imaging
Core of the NIDDK-funded Cleveland Digestive Diseases Research Core Center (DDRCC). A centralized core
component that randomly samples and re-scores intestinal sections from PPG experimental mice across all three
projects and across a time-course of ages will provide a common framework for PPG quality control, as well as
assist greatly in assuring standardized interpretation of data generated by each of the three projects. A strength
of this component is the availability of an experienced GI pathologists, Dr. Xin, to perform the histopathological
re-scoring and to provide ongoing consultative service to the projects concerning the pathological features of
disease in this model. Dr. Pizarro will continue to direct the core. A committee consisting of the core director and
project leaders will address routine quality control and prioritization issues. An oversight committee will review
the operation of the core on a yearly basis.

## Key facts

- **NIH application ID:** 9995028
- **Project number:** 5P01DK091222-10
- **Recipient organization:** CASE WESTERN RESERVE UNIVERSITY
- **Principal Investigator:** Theresa Torres Pizarro
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $237,750
- **Award type:** 5
- **Project period:** 2011-08-01 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9995028

## Citation

> US National Institutes of Health, RePORTER application 9995028, Mouse Congenics Core (5P01DK091222-10). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9995028. Licensed CC0.

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