# Training in isotopic techniques for metabolic research

> **NIH NIH R25** · VANDERBILT UNIVERSITY · 2020 · $104,773

## Abstract

Project Summary
This application is in response to Funding Opportunity Announcement PAR-15-139 a five day
course entitled” Isotope Tracers in Metabolic Research: Principles and Practice in Kinetic
Analysis”. The course faculty will present state of the art methods for using both radioactive and
stable isotopes to investigate whole body and organ metabolism in vivo and intracellular flux
rates and pathway regulation in vivo and in vitro. The basic aspects of modeling will be
considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism
and energy balance. Theoretical and practical matters related to sample analysis by mass
spectrometry and NMR will be discussed, including detailed numerical examples of calculations
involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures
will discuss in more detail the use of positional and mass isotopomer analysis for intracellular
flux rates and various aspects of protein and amino acid metabolism. Specific applications
(hyperinsulinemic euglycemic clamp) for use in humans and animals will be presented. The
course uses a lecture format combined with problem discussions. In addition a popular aspect
of the course is that trainees meet one on one with course faculty to discuss their specific
research project and can present their project in an evening session. A new addition is detailed
theory and experimental protocols to monitor specific pathways in a webinar style format that
will be available on the course website to the entire research community. Typically, 75-85
trainees attended the course each year. Feedback from attendees has been very laudatory. A
number of trainees have developed new research projects using isotope technologies. Thus
this popular course builds on and reinforces fundamental skills needed to study metabolic
processes that are relevant to the mission of NIDDK.

## Key facts

- **NIH application ID:** 9996570
- **Project number:** 5R25DK111362-03
- **Recipient organization:** VANDERBILT UNIVERSITY
- **Principal Investigator:** OWEN P MCGUINNESS
- **Activity code:** R25 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $104,773
- **Award type:** 5
- **Project period:** 2018-09-13 → 2023-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9996570

## Citation

> US National Institutes of Health, RePORTER application 9996570, Training in isotopic techniques for metabolic research (5R25DK111362-03). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9996570. Licensed CC0.

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