# Translation Studies of the Planet Specific Receptor Trem Like Transcript (TLT)

> **NIH NIH R01** · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · 2020 · $372,500

## Abstract

Project Summary
According to the World Health Organization, Cardiovascular disease (CVD) is responsible for
31% of deaths worldwide making it the largest global cause of mortality. These numbers are
reflected in the United states where over 600,000 people died of CVD. CVD is an inflammatory
disease that often leads to mortality by thrombosis and platelets mediate both the inflammatory
and thrombotic aspects of CVD. Our laboratory studies the Triggering receptor expressed in
myeloid cells or (TLT-1) that is stored in the granules of the platelets and is both expressed to
the platelet surface and released after platelet activation. TLT-1 binds fibrinogen. While TLT-1
has demonstrated roles in Cardiovascular disease, sepsis, and acute lung injury, its interaction
with fibrinogen is poorly understood. We hypothesize that TLT-1’s interaction with fibrinogen
is a major pathway by which the immune system commandeers the hemostatic system for
immune function. In this application we propose to mechanistically define this interaction and
demonstrate is usefulness as a therapeutic target. We propose the following three aims: Aim I –
Determine the relative contribution of fibrinogen binding to TLT-1 and IIb2 using double
deficient (treml1-/-/itga2b-/-) mice; Aim II - Elucidate TLT-1 signaling pathways; Aim III –
Evaluate the effect of TLT-1 prophylactics on the progression of CVD and obesity. At the
completion of these aims we will have defined TLT-1’s contribution to platelet interaction with
fibrinogen and is potential as a therapeutic target.

## Key facts

- **NIH application ID:** 9996765
- **Project number:** 5R01HL090933-07
- **Recipient organization:** UNIVERSITY OF PUERTO RICO RIO PIEDRAS
- **Principal Investigator:** A. Valance Washington
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $372,500
- **Award type:** 5
- **Project period:** 2011-06-16 → 2021-02-01

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9996765

## Citation

> US National Institutes of Health, RePORTER application 9996765, Translation Studies of the Planet Specific Receptor Trem Like Transcript (TLT) (5R01HL090933-07). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9996765. Licensed CC0.

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